Merck

Twenty years of bacterial genome sequencing.

Nature reviews. Microbiology (2015-11-10)
Nicholas J Loman, Mark J Pallen
ABSTRACT

Twenty years ago, the publication of the first bacterial genome sequence, from Haemophilus influenzae, shook the world of bacteriology. In this Timeline, we review the first two decades of bacterial genome sequencing, which have been marked by three revolutions: whole-genome shotgun sequencing, high-throughput sequencing and single-molecule long-read sequencing. We summarize the social history of sequencing and its impact on our understanding of the biology, diversity and evolution of bacteria, while also highlighting spin-offs and translational impact in the clinic. We look forward to a 'sequencing singularity', where sequencing becomes the method of choice for as-yet unthinkable applications in bacteriology and beyond.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
D-Pantothenic acid hemicalcium salt, ≥98.0%
Sigma-Aldrich
Calcium pantothenate, meets USP testing specifications, monograph mol wt. 476.53 (C18H32CaN2O10)
Sigma-Aldrich
D-Pantothenic acid hemicalcium salt, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture