Merck

HPLC Analysis of Glutamine Enantiomers on Astec® CHIROBIOTIC® T (23 °C)

HPLC Analysis of Glutamine Enantiomers on Astec® CHIROBIOTIC® T (23 °C) application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Astec® CHIROBIOTIC® T Chiral HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

standard

Product No.
Description
Pricing

L-Glutamine

meets USP testing specifications, suitable for cell culture, 99.0-101.0%, from non-animal source

L-Glutamine

ReagentPlus®, ≥99% (HPLC)

L-Glutamine

γ-irradiated, BioXtra, suitable for cell culture

D-Glutamine

≥98% (HPLC)

L-Glutamine solution

200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture

L-Glutamine solution

200 mM, Hybri-Max, sterile-filtered, suitable for hybridoma

L-Glutamine

CONDITIONS

column

CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

[A] water; [B] ethanol; (50:50, A:B)

flow rate

1 mL/min

column temp.

23 °C

detector

UV, 205 nm

injection

5 μL

sample gas

1 mg/mL in methanol

Description

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC