Merck

HPLC Analysis of Glutamine Enantiomers on Astec® CHIROBIOTIC® T

HPLC Analysis of Glutamine Enantiomers on Astec® CHIROBIOTIC® T application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Astec® CHIROBIOTIC® T Chiral HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

guard cartridge

Product No.
Description
Pricing

Astec® CHIROBIOTIC® T Chiral HPLC Guard Column

Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm

standard

Product No.
Description
Pricing

L-Glutamine

meets USP testing specifications, suitable for cell culture, 99.0-101.0%, from non-animal source

L-Glutamine

ReagentPlus®, ≥99% (HPLC)

L-Glutamine

γ-irradiated, BioXtra, suitable for cell culture

D-Glutamine

≥98% (HPLC)

L-Glutamine solution

200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture

L-Glutamine

L-Glutamine

BioUltra, ≥99.5% (NT)

L-Glutamine

L-Glutamine

certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

CONDITIONS

column

CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

[A] water; [B] methanol; [C] formic acid; (30:70:0.02, A:B:C)

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 205 nm

injection

10 μL

sample

300 μg /mL in 30:70 water:methanol

Description

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC