Merck

HPLC Analysis of Salicylic Acid and Acetylsalicylic Acid (Aspirin) on Ascentis® Express OH5

HPLC Analysis of Salicylic Acid and Acetylsalicylic Acid (Aspirin) on Ascentis® Express OH5 application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Ascentis® Express OH5, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 3 mm

standard

Product No.
Description
Pricing

Salicylic acid

meets analytical specification of Ph. Eur., BP, USP, 99.5-100.5% (calc. to the dried substance)

CONDITIONS

column

Ascentis Express OH5, 10 cm x 3.0 mm I.D., 2.7 μm particles (53769-U)

mobile phase

2 mM ammonium acetate in 10:90 water:acetonitrile, pH to 7.0 with acetic acid, glacial

flow rate

0.5 mL/min

pressure

850 psi (59 bar)

column temp.

35 °C

detector

UV, 235 nm

injection

2 μL

sample

100 μg/mL salicylic acid, 200 μg/mL acetylsalicylic acid in methanol:acetonitrile (3:7)

Description

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany