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HomeSingle Molecule Counting (SMC®) TechnologyThe SMCxPRO® Immunoassay Platform for Ultrasensitive Biomarker Detection

The SMCxPRO® Immunoassay Platform for Ultrasensitive Biomarker Detection

When the success of your research requires identifying and quantifying low-level proteins, you need technology you can rely on to detect your target. The compact SMCxPRO® immunoassay platform provides fast ultrasensitive biomarker detection.

What is the SMCxPRO® Platform?

The SMCxPRO® platform is an ultrasensitive immunoassay platform that can detect low levels of established disease biomarkers, capturing extremely small concentrations. As a result, this platform can monitor small changes in protein concentrations to accurately measure biomarkers associated with disease progression for research purposes. In addition, the small sample volume requirements allow researchers to conserve valuable samples, reduce program costs, and improve productivity. This affordable, fast, and compact benchtop instrument allows research labs, including ones on a budget, to access reliable protein detection and expand the quest for drug discovery research and development.

Using Single Molecule Counting (SMC®) technology, this protein detection platform offers the ability to take the analysis of proteins down to femtogram/mL levels. This improved level of sensitivity allows for previously undetectable proteins to now be quantified, including endogenous levels.

SMCxPRO® Ultrasensitive Protein Detection Platform

Image of the SMCxPRO® instrument that is part of the ultrasensitive protein and biomarker detection platform.

Figure 1.The SMCxPRO® biomarker detection instrument.

Benefits include:

  • High sensitivity (femtogram/mL) quantification
  • Minimal bench space: 14” W x 16” H x 17.5” D
  • Affordable
  • Bead-based kits and plate-based kits
  • Custom assay and sample testing services
  • Software is 21CFR part II compliant and LIMS compatible

SMCxPRO® Platform vs. Erenna® Platform Comparison

SMC® technology has utilized the Erenna® instrument for years as a proven, reliable instrument for high sensitivity protein measurement. The SMCxPRO® platform expands the portfolio to provide a compact, affordable option to achieve the same high sensitivity. Figure 2 shows a comparison of the SMCxPRO® platform vs the Erenna® platform.

SMCxPRO® Platform vs. Erenna® Platform Comparison

Figure 2.Individual and pooled plasma samples from four SMC® immunoassay kits were analyzed on both the SMCxPRO® and Erenna® platforms. For all four analytes measured, the mean interpolated concentration of each sample (R2) was nearly identical between the two instruments. Human cTnI (03-0092-00), R2=0.9944, Human IL-6 (03-0089-01), R2=0.9972, Human IL-4 (03-0052-00), R2=0.9976, and Human IL-23 (03-0112-00) R2=0.9413.

Take Multiplexing Results Even Further

Multiplexing allows you to screen multiple markers quickly and easily. The SMCxPRO® platform allows researchers to take this even further, to the femtogram/mL level, with a seamless transition from platform to platform.

MILLIPLEX® multiplex assays and the SMCxPRO® platform cover the complete quantitative measurement of diseased states for research purposes. This improved sensitivity range allows for early recognition of marker changes which will ultimately improve research for diagnostic and patient care.

Figure 3 and Figure 4 describe an example of combining multiplexing and high sensitivity immunoassays in cardiac research.

Multiplex and High Sensitivity Analysis of cTnI

Multiplex and High Sensitivity Analysis of cTnI

Figure 3.A combination of the MILLIPLEX® Human cTnI (HCVD1MAG-67K) and SMCxPRO® Human cTnI (03-0092-00) assays provide a 7-log range of detection. This allows for the exploration of patient samples both pre- and post-myocardial infarction. Diagnostic cut-off range was determined from literature review.1

Multiplex and High Sensitivity Analysis of IL-6

Multiplex and High Sensitivity Analysis of IL-6

Figure 4.Both the MILLIPLEX® Human IL-6 (HSTCMAG-28SK) and the SMCxPRO® Human IL-6 (03-0089-01) assays provide detection and resolution of the cytokine within a study population. The combination of platforms allows for the detection and exploration of the biomarker on a multiplex platform, with detailed detection and resolution of the underlying changes in the marker by an ultrasensitive technology. Data was generated using the Erenna® platform. Reference range was determined from literature review.2 * As defined in a population of: Normal N =125 40-65 years old and Heart Failure N = 32 age matched.

Grant Application Support

Access the necessary background and technology information to be easily incorporated into a grant application for the SMCxPRO® platform. From text to data, this comprehensive SMCxPRO® Grant Accelerator Package can quickly assist you with any funding application.

SMCxPRO® Instrument Service Plans and Support

The SMCxPRO® platform provides a highly sensitive, affordable, and easy-to-use protein quantification solution for your research. This instrument is backed by expert technical support and advanced processes to ensure smooth operation, reliability, and consistency.

Service Plans

Get the most out of your SMCxPRO® platform. Choose one of our best-in-class service plans, and be confident that your system is always in excellent condition and able to meet your needs while ensuring the longevity of your instrument.

We offer a wide range of service agreements and preventative maintenance plans, that are supported by highly trained service professionals and technical support representatives. As part of our complete solution, our service agreements are structured, yet flexible, allowing you to select the level of support that you prefer.

Advantages of Maintaining a Service Plan

  • Maintain optimal instrument performance to ensure high-quality data
  • Planned instrument maintenance reduces overall service costs
  • Best protection for your instrument investment and its long-term operation
Table 1. SMCxPRO® service plans.

One-Year Warranty

The standard one-year warranty with the purchase of the SMCxPRO® instrument covers the replacement of any components that may be damaged through normal use of the instrument within the first year of installation. Qualified field service engineers and/or application specialists provide instrument installation upon purchase and either a field service engineer or application specialist will demonstrate how to maintain the instrument as part of customer training.

Contracts for service assume continuous warranty or service contract coverage. If coverage lapses and is later re-established, the instrument must first be restored to factory specifications and may result in additional costs.

Instrument Technical Support

In addition to onsite service support, our worldwide technical support team can assist you anytime by email or telephone. Their wide range of technical expertise enables them to address questions on both hardware and software. If your concern cannot be resolved by our technical support team, we will schedule an onsite visit from a trained service engineer or application specialist.

SMCxPRO® Instrument Registration

Register your SMCxPRO® instrument to receive information and updates on your instrument's software and firmware as they become available.

Related Products and Services

However you wish to build your experiment, we provide you with experienced technical support to advance your research every step of the way. In addition to our portfolio of kits, we offer affordable hands-on custom assay development training to help you develop an assay to your own protein of interest.

We also offer qualification services to verify and ensure your instrument is working according to the manufacturer’s specifications. These services are performed at the time of instrument installation.

Ancillary Equipment and Software

For Research Use Only. Not For Use In Diagnostic Procedures.

References

1.
Panteghini M. 2003. Recommendations on Use of Biochemical Markers in Acute Coronary Syndrome: IFCC Proposals. EJIFCC. 14(2):104-108. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6169146/
2.
Todd J, Simpson P, Estis J, Torres V, Wub AH. 2013. Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays. Cytokine. 64(3):660-665. http://dx.doi.org/10.1016/j.cyto.2013.09.018