HomeProtein & Nucleic Acid InteractionsUsing Duolink® in Protein Interaction Studies

Using Duolink® in Protein Interaction Studies

The video above follows the simple and straightforward procedure that allows you to detect, quantify and obtain cell localization of protein interactions and their modifications in a single experiment.

  • Cells or tissue deposited on slides or in microplates are fixed to preserve activation status and transient interactions.
  • Validated primary antibodies for the targets are added followed by binding of the PLA probes.
  • Hybridization of the oligonucleotide arms of the PLA probes will create a template for rolling circle amplification (RCA) only when the epitopes of the target proteins are in close proximity (<40 nm)
  • Amplification and labeling of the RCA product by detection probes.
  • The resulting spot can be detected and visualized by standard microscopy.
  • Images can easily be quantitatively analyzed using the Duolink Image Tool software, which facilitates both average and single cell data analysis.
Sign In To Continue

To continue reading please sign in or create an account.

Don't Have An Account?