These products are available in the US, Canada, and select European countries.
Pericytes are multipotent mesenchymal-like cells found in association with the walls of small blood vessels. They are important for angiogenesis, for the structural integrity of the microvasculature, and for the regulation of blood flow. However, they can also develop into malignant tumors called hemangiopericytomas. To date, these cells have demonstrated the ability to differentiate into adipocytes, osteoblasts, fibroblasts, smooth muscle cells, and phagocytes (macrophages).
PromoCell® Pericytes are derived from normal human placenta. Shortly after isolation, all Pericytes are cryopreserved at passage 2 (P2) using proprietary, serum-free freezing medium, Cryo-SFM. Each cryovial contains at least 500,000 viable pericytes after thawing. Human pericytes from placenta are also available as proliferating cell cultures generated from cryopreserved cells that have been thawed and cultured for three days.
Pericyte Growth Medium was developed for the in vitro cultivation of human pericytes from all tissues. The Pericyte Growth Medium consists of a 500 mL bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium constitutes the complete Growth Medium.
Pericyte Growth Medium contains all components necessary for the optimal growth of human pericytes. Pericyte Growth Medium does not contain antibiotics or antimycotics and is formulated for the use in an incubator with an atmosphere of 5% CO2.
Rigid quality control tests are performed for each lot of PromoCell® Pericytes.
All lots of PromoCell® Pericyte Growth Medium are subjected to comprehensive quality control tests using primary human placenta-derived pericytes. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested pericytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Intended Use of Primary Pericytes
These products are for in vitro use only and not for diagnostic or therapeutic procedures. For safety precautions please see appropriate MSDS.
Note: Due to their low serum content or the absence of serum, PromoCell® media are not suitable for trypsin neutralization (when subculturing the cells). Instead we recommend using our DetachKit (C-41200, C-41210, C-41220), which contains HEPES BSS, Trypsin/EDTA and Trypsin Neutralizing Solution.
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