Merck

04-1569

Sigma-Aldrich

Anti-RNA polymerase II subunit B1 Antibody, clone 4F8

clone 4F8, from rat

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別名:
DNA-directed RNA polymerase II A, DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA polymerase II subunit B1, RNA-directed RNA
eCl@ss:
32160702
NACRES:
NA.41

由来生物

rat

品質水準

抗体製品の状態

purified antibody

antibody product type

primary antibodies

クローン

4F8, monoclonal

species reactivity

mouse

species reactivity (predicted by homology)

human (based on 100% sequence homology)

technique(s)

ChIP: suitable
ELISA: suitable
western blot: suitable

アイソタイプ

IgG1κ

NCBIアクセッション番号

UniProtアクセッション番号

輸送温度

wet ice

target post-translational modification

unmodified

Gene Information

human ... POLR2B(5431)

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当該品目
ABE3004-157004-1571
antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

4F8, monoclonal

clone

polyclonal

clone

4E12, monoclonal

clone

3E10, monoclonal

species reactivity

mouse

species reactivity

human

species reactivity

mouse

species reactivity

mouse

technique(s)

ChIP: suitable, ELISA: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

ChIP: suitable, ELISA: suitable, western blot: suitable

technique(s)

ChIP: suitable, ELISA: suitable, western blot: suitable

isotype

IgG1κ

isotype

-

isotype

IgG1κ

isotype

IgG1κ

詳細

RNA polymerase II subunit B1 (RPB1) is the largest subunit of the RNA polymerase II complex. As a holoenzyme RNA polymerase II catalyzes transcription of eukaryotic DNA into RNA using the four ribonucleoside triphosphates as substrates. The RBP1 subunit, in combination with other polymerase subunits, forms a large central cleft that maintains contact between the active site of the enzyme, the DNA template, and the nascent RNA transcript. This subunit also contains a carboxy terminal domain (CTD) consisting of tandem heptapeptide repeats. In actively transcribing RNA polymerase ‘Ser-2’ and ‘Ser-5’ of the heptapeptide repeat are phosphorylated. Phosphorylation activates the RNA polymerase II beta subunit, allowing it to serve as an assembly platform for additional subunits that modulate initiation, elongation, termination and mRNA processing.

特異性

This antibody recognizes RNA polymerase II subunit B1.

免疫原

Epitope: Unknown
Ovalbumin-conjugated linear peptide corresponding to human RNA polymerase II subunit B1.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

エピジェネティクス及び核内機能分子
Research Sub Category
転写因子

RNA代謝及び結合タンパク質
Anti-RNA polymerase II subunit B1 Antibody, clone 4F8 is a Rat monoclonal antibody for detection of RNA polymerase II subunit B1 also known as DNA-directed RNA polymerase II A & has been validated in WB, ELISA.
Chromatin Immunoprecipitation Analysis: A representative lot was used by independent laboraotry in ChIP. (Chapman, R., et al. (2007). Science. 318(5857):1780 -1782.)

品質

Evaluated by Western Blot in NIH/3T3 cell lysates.

Western Blot Analysis: 1 µg/ml of this antibody detected RNA polymerase II subunit B1 on 10 µg of NIH/3T3 cell lysates.

ターゲットの説明

~ 220 kDa

物理的形状

Protein G Purified
Format: Purified
Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
NIH/3T3 cell lysates

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

保管分類コード

12 - Non Combustible Liquids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable


Certificates of Analysis (COA)

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COAをリクエストする

Rob D Chapman et al.
Science (New York, N.Y.), 318(5857), 1780-1782 (2007-12-15)
RNA polymerase II is distinguished by its large carboxyl-terminal repeat domain (CTD), composed of repeats of the consensus heptapeptide Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Differential phosphorylation of serine-2 and serine-5 at the 5' and 3' regions of genes appears to coordinate the localization of
Joshua E Mayfield et al.
eLife, 8 (2019-08-07)
The Positive Transcription Elongation Factor b (P-TEFb) phosphorylates Ser2 residues of the C-terminal domain (CTD) of the largest subunit (RPB1) of RNA polymerase II and is essential for the transition from transcription initiation to elongation in vivo. Surprisingly, P-TEFb exhibits
Wenyu Wang et al.
Nature communications, 12(1), 4441-4441 (2021-07-23)
BRD4, a Bromodomain and Extraterminal (BET) protein family member, is a promising anti-cancer drug target. However, resistance to BET inhibitors targeting BRD4 is common in solid tumors. Here, we show that cancer-associated fibroblast (CAF)-activated stromal signaling, interleukin-6/8-JAK2, induces BRD4 phosphorylation

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