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Merck

1.05642

Supelco

HPTLC plates, Silica gel 60 F₂₅₄

pkg of 50 plates, plate L × W 20 cm × 10 cm, glass support

別名:

HPTLC Silica Gel Plates

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About This Item

UNSPSCコード:
41115711
NACRES:
NB.21

物質

glass support
silica gel 60 matrix

品質水準

特徴

binder Organic Polymer
fluorescent indicator

包装

pkg of 50 plates

テクニック

thin layer chromatography (TLC): suitable

層厚

200 μm

プレートL × W

20 cm × 10 cm

粒径

5-6 μm

ポアサイズ

60 Å medium pore diameter

保管温度

2-30°C

詳細

50 Glass plates 20 x 10 cm
HPTLC plates deliver fast and quantitative analysis of complex samples for manual or automated use. Our HPTLC silica plates work three times faster than conventional TLC plates – and they are more sensitive. The particle sizes range in HPTLC is between 4-8 µm, which creates a smoother surface and a higher separation power than TLC plates.

アプリケーション

Glass HPTLC Silica gel 60 F₂₅₄ plates. Silica gel HPTLC plates size 20 x 10 cm, 50 sheets.

関連事項

Replaces: 5642-6; 5642

アナリシスノート

Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 5 - 7 µm
Layer thickness: 150 - 200 µm
Deviation of layer thickness per plate: ≤ 35 µm
Chromatographic testings:
A) Colour test
hRf-values
- bleu vif organol, colour test, lipophile:
17 - 22
- Ceres Black G, colour test, lipophile: 38 - 43
- ceres Violet brn, colour test, lipophile: 58 - 64
separation number
- colourtest, lipophile:
≥ 10.0
B) Steroid test
hRf-values
- hydrocortisone, steroid test:
23 - 29
- Reichstein S, steroid test: 36 - 45
- methyltestosterone, steroid test: 43 - 52
separation number
- steroid test:
≥ 10.5
Typical value determined on a conditioned plate
Eluent A) Toluene (45% rel.humidity)
Eluent B) Ethyl acetate/Toluene (95+5 v/v; 20% rel.humidity)

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資料

Learn about our recently launched HPTLC calibration mix, developed in collaboration with CAMAG, that enables for an efficient and reliable universal system suitability testing (SST).

An HPTLC method for rapid urea detection in milk using a TLC plate scanner and Silica Gel 60 F254 HPTLC plate.

Development of an HPTLC based method, using Silica Gel 60 F254 plate, to determine Betamethasone Sodium Phosphate over a concentration range of 1 to 7 µg/spot.

In this article we present several HPTLC applications and analytical standards for ginsenosides.

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プロトコル

HPTLC was used to analyze caffeine in coffee. Filtration of coffee through a PTFE syringe filter was the only sample preparation. Quantitation was conducted with a TLC scanner.

In the present study we demonstrate compliance of silica gel 60 F254 HPTLC plates with USP requirements, and how HPTLC plates are suitable for the Sildenafil citrate-Limit of Imidazole test.

Powdered black pepper tested for pierine content follows USP guidelines using HPTLC and HPLC for dietary supplements.

HPTLC fingerprinting enables rapid identification of passion flower with related reference materials.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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