Proteinase K

Name: Proteinase K
Brand: MM

(from Tritirachium album) solution in Tris/HCl pH 7.5; 0.01 mol/l; 600 mAnson-U/ml; for molecular biology EC 3.4.21.14

別名:

ProK, native proteinase K solution, non-specific protease

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この商品について

NACRES:

NA.51

UNSPSC Code:

12352204

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製品名

Proteinase K, (from Tritirachium album) solution in Tris/HCl pH 7.5; 0.01 mol/l; 600 mAnson-U/ml; for molecular biology EC 3.4.21.14

form

liquid

pH

7.5 (25 °C in H₂O, undiluted)

density

1.10 g/cm³ at 20 °C

storage temp.

2-8°C

Quality Level

400

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当該品目	1.24568	RPROTKSOL-RO	RPROTK-RO
Sigma-Aldrich 1.07393 Proteinase K	Sigma-Aldrich 1.24568 Proteinase K	Roche RPROTKSOL-RO Proteinase K, recombinant, PCR Grade	Roche RPROTK-RO Proteinase K, recombinant, PCR Grade
form liquid	form solid	form buffered aqueous solution (18 ± mg/mL; pH 7.5)	form lyophilized
pH 7.5 (25 °C in H₂O, undiluted)	pH 6.2-6.8 (20 °C, 10 g/L in H₂O)	pH -	pH -
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
Quality Level 400	Quality Level 400	Quality Level 100	Quality Level 100
density 1.10 g/cm³ at 20 °C	density 1.1 g/cm³	density -	density -

Analysis Note

Appearance (colour): colourless
Appearance (clearness): clear
Activity (hemoglobin; pH 7.5; 37 °C): ≥ 600 mAnsonU/ml
Spec. activity (calc. on protein): ≥ 40.0 mAnsonU/mg
DNases (Nicking activity; pBR 322; 6 h; 37 °C): not detectable
RNases (RNA; 2 h; 37°C): not detectable
Colony count (aerobic bacteria): ≤ 10 CFU/ml

Application

Proteinase K has been used:

to de-crosslink immunoprecipitated samples
to treat the poly-L-lysine coated slides of colon tissues for terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay
in in situ hybridization

Biochem/physiol Actions

Proteinase K catalyzes the hydrolysis of esters and peptide bonds. It is used with non-aqueous hydrated solvents for synthesizing peptides. Proteinase K is used to break the cross-linking that develops secondary to formalin fixation and expose the target sequence for primers and polymerase.

General description

Proteinase K is an endopeptidase that belongs to the subtilisin family of proteinases. The polypeptide chain contains 278 amino acids with the catalytic triad Asp39, His69, Ser224.

Hazard Classifications

Resp. Sens. 1

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

保管分類

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

試験成績書（COA）

製品のロット番号・バッチ番号を入力して、試験成績書（COA）を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

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Purification, characterization, gene cloning, sequencing, and overexpression of aminopeptidase N from Streptococcus thermophilus A.

F Chavagnat et al.

Applied and environmental microbiology, 65(7), 3001-3007 (1999-07-02)

The general aminopeptidase PepN from Streptococcus thermophilus A was purified to protein homogeneity by hydroxyapatite, anion-exchange, and gel filtration chromatographies. The PepN enzyme was estimated to be a monomer of 95 kDa, with maximal activity on N-Lys-7-amino-4-methylcoumarin at pH 7

Early Diverging Fungus Mucor circinelloides Lacks Centromeric Histone CENP-A and Displays a Mosaic of Point and Regional Centromeres.

María Isabel Navarro-Mendoza et al.

Current biology : CB, 29(22), 3791-3802 (2019-11-05)

Centromeres are rapidly evolving across eukaryotes, despite performing a conserved function to ensure high-fidelity chromosome segregation. CENP-A chromatin is a hallmark of a functional centromere in most organisms. Due to its critical role in kinetochore architecture, the loss of CENP-A

資料

Best Product & Supplier for Proteinase K

Balancing Proteinase K cost with quality and technical support ensures optimal enzyme selection for diverse applications.

Guide on How to Use Proteinase K in Different Procedures

Guidelines on use of proteinase K, an enzyme commonly used to degrade proteins, and protect DNA and RNA from degradation in samples.

How Proteinase K Can Be Used in Blood DNA Extraction Applications

In blood DNA extraction, Proteinase K, an enzyme commonly used to degrade proteins, can help break down the cellular and nuclear membranes, releasing DNA from the cells that protect it from degradation and increase purity/yield making it more suitable for various molecular biology techniques.

Proteinase K: Introduction & Applications

Proteinase K aids in molecular biology applications by digesting structural proteins, removing nucleases, and isolating intact genomic DNA.

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