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ABTS^™ Chromophore

• 別名: ABTS^™ Chromophore
• 実験式（ヒル表記法）: C₁₈H₁₈N₄O₆S₄
• 分子量: 514.62
• UNSPSCコード: 41116133
• NACRES: NA.41

特性

• 形状: solution
• 品質水準: 100
• 含みます: ≤0.1% sodium azide as preservative
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• 色: clearvery pale yellow
• 輸送温度: ambient
• 保管温度: 2-8°C

詳細

詳細

Peroxidase substrate that is soluble, safe, convenient and has good overall performance in ELISA. ABTS^™ Chromophore develops intense blue-green color that can be measured at 405 nm to 410 nm. May be used for both kinetic and endpoint reactions.

Single component peroxidase substrate which is soluble, safe, convenient and has good overall performance for ELISA. In the presence of horseradish peroxidase (HRP) and hydrogen peroxide, ABTS is oxidized to an intense blue-green, water-soluble product. This product is a radical cation having an absorption maximum at 416 nm. Typically, product formation is measured spectrophotometrically at 405 nm, allowing a quantitative analysis of HRP-based systems. The color formation as a function of time may be monitored, or the reaction may be stopped by the addition of acid; since the acid does not alter the absorbance at 405 nm, one may perform both kinetic and endpoint measurements at the same wavelength.

警告

Toxicity: Irritant (B)

物理的形状

Contains optimal concentrations of ABTS and H₂O₂ in a citrate buffer (pH 4.0) with proprietary stabilizers.

再構成

ABTS^™ solution should be stored at 4°C, but must be warmed to room temperature before use. The solution should be protected from exposure to direct sunlight, and should be discarded if it becomes bright yellow or turbid.

その他情報

Rodgriquez-Lopez, J.N., et al. 2000. Biochemistry39, 13201.

法的情報

ABTS is a trademark of Boehringer Mannheim Corp.

ABTS is a trademark of Roche

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable