616464

InSolution TGF-β RI Kinase Inhibitor VI, SB431542

The InSolution TGF-β RI Kinase Inhibitor VI, SB431542 controls the biological activity of TGF-β RI. Primarily used for Phosphorylation & Dephosphorylation applications.

• Synonym(s): InSolution TGF-β RI Kinase Inhibitor VI, SB431542
• Empirical Formula (Hill Notation): C₂₂H₁₆N₄O₃ · 2H₂O
• CAS Number: 301836-41-9
• Molecular Weight: 420.42

Properties

• 品質水準: 100
• アッセイ: ≥97% (HPLC)
• 形状: liquid
• manufacturer/tradename: Calbiochem^®
• 保管条件: OK to freeze; desiccated (hygroscopic); protect from light
• 輸送温度: ambient
• 保管温度: −20°C
• InChI: 1S/C22H16N4O3/c23-21(27)13-4-6-14(7-5-13)22-25-19(20(26-22)16-3-1-2-10-24-16)15-8-9-17-18(11-15)29-12-28-17/h1-11H,12H2,(H2,23,27)(H,25,26)
• InChI key: FHYUGAJXYORMHI-UHFFFAOYSA-N

Description

詳細

A cell-permeable triarylimidazole compound that is shown to effectively inhibit cellular Smad2 phosphorylation (>90% inhibition by 10 µM inhibitor) upon vector-mediated expression of constitutively active ALK4, ALK5, or ALK7 in NIH 3T3 cells, while exhibiting little effect against Smad1 phosphorylation by other members of type I receptors for TGF-β in NIH 3T3 cultures expressing active ALK1, 2, 3, or 6. When tested directly in cell-free kinase assays, SB431542 is demonstrated to potently inhibit the activity of ALK4 and ALK5 (IC₅₀ = 140 nM and 94 nM, respectively) with no or much reduced potency toward a panel of 24 other kinases (IC₅₀ ≥10 µM in the presence of 10 µM ATP), including ALK2 and ALK6. Reported to improve the efficiency of 4-TF-induced human iPSCs generation from fibroblast cultures by >200-fold when used together with PD0325901 (Cat. No. 444966) and Thiazovivin (Cat. No. 420220).

包装

Packaged under inert gas

警告

Toxicity: Irritant (B)

物理的形状

A 100 mM (5 mg/119 µl) solution of SB431542 (Cat. No. 616461) in DMSO.

再構成

Following initial thaw, aliquot and freeze (-20°C).

その他情報

Ikushima, H., et al. 2009. Cell Stem Cell5, 504.
Lin, T., et al. 2009. Nat. Methods6, 805.
Maherali, N. and Hochedlinger, K., 2009. Curr. Biol.19, 1718.
Callahan, J.F., et al. 2002. J. Med. Chem.45, 999.
Inman, G.J., et al. 2002. Mol. Pharmacol.62, 65.
Laping, N.J., et al. 2002. Mol. Pharmacol.62, 58.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): 188.6 °F - (refers to pure substance)
• 引火点(℃): 87 °C - (refers to pure substance)