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71110-M

rLysozyme Solution

Name: rLysozyme Solution
Brand: MM

Stabilized recombinant lysozyme

別名:

recombinant lysozyme

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この商品について

NACRES:

NA.77

UNSPSC Code:

12352202

Form:

solution

Recombinant:

expressed in E. coli

この製品は、Merck Milliporeウェブサイトでのみ注文できます。

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特性

recombinant

expressed in E. coli

Quality Segment

400

form

solution

manufacturer/tradename

Novagen^®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

input

bacteria and yeast sample(s)

storage temp.

−20°C

詳細

General description

rLysozyme Solution contains a highlypurified and stabilized recombinant lysozymethat can be used for lysis of Gram-negativebacteria, such as E. coli. The enzyme catalyzes the hydrolysis of N-acetylmuramide linkages in bacterial cell walls. The specific activity of rLysozyme (1700 KU/mg) for E. coli lysis is 250 times greater than that of chicken egg white lysozyme. rLysozyme is optimally active at physiological pH. Very small amounts of rLysozyme (3–5 KU/g cell paste) enhance the efficiency of protein extraction withBugBuster, BugBuster HT, and PopCultureReagents. In the absence of protein extractionreagents, direct lysis of E. coli can be achieved by treatment of 1.0 gram cell paste with 45–60 KU rLysozyme. The product is supplied as a ready-to-use solution at a concentration range of 27–33 KU/µl in 50% glycerol containing 50 mM Tris-HCl, 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% TRITON X-100, pH 7.5. rLysozyme Solution is stable at –20°C.

Other Notes

One unit of rLysozyme is defined as the amount of enzyme necessary to cause a decrease of 0.025 A₄₅₀ unit per min at 25°C in a 1-ml suspension (1 mg/ml) of Tuner(DE3) cells in 0.5 x BugBuster diluted with 50 mM Tris-HCl, pH 7.5.

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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当該品目	SAE0152	L1667	L3790
Millipore 71110M rLysozyme Solution	Sigma-Aldrich SAE0152 リゾチームニワトリ卵白由来	Sigma-Aldrich L1667 リゾチームヒト	Sigma-Aldrich L3790 リゾチームニワトリ卵白由来
description Stabilized recombinant lysozyme	description free of DNA contaminants, suitable for Microbiome research, lyophilized powder, protein ≥90%, ≥39,000 units/mg protein	description Lysobac^™, recombinant, expressed in rice, lyophilized powder, ≥100,000 units/mg protein	description 10 mg/mL
recombinant expressed in E. coli	recombinant -	recombinant expressed in rice	recombinant -
form solution	form powder	form lyophilized powder	form liquid
storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C
storage condition OK to freeze, avoid repeated freeze/thaw cycles	storage condition -	storage condition -	storage condition -
manufacturer/tradename Novagen^®	manufacturer/tradename -	manufacturer/tradename -	manufacturer/tradename -
Quality Level 400	Quality Level 100	Quality Level 200	Quality Level 200

保管分類

10 - Combustible liquids

wgk

WGK 2

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