A-003-M

Poly-D-Lysine solution, 1.0 mg/mL

The Poly-D-Lysine solution promotes the adhesion of tissues/sections to the culture vessel.

• Synonym(s): poly lysine
• eCl@ss: 32160801
• NACRES: NA.75

Properties

• 品質水準: 100
• 形状: liquid
• manufacturer/tradename: Specialty Media
• technique(s): cell culture | mammalian: suitable
• input: sample type neural stem cell(s); sample type epithelial cells; sample type: human embryonic stem cell(s); sample type hematopoietic stem cell(s); sample type mesenchymal stem cell(s); sample type pancreatic stem cell(s); sample type induced pluripotent stem cell(s)

Description

詳細

Poly-D-Lysine promotes the adhesion of tissues/sections to the culture vessel. The polycationic property of the molecule allows interaction with anionic sites on the tissue/cells promoting effective attachment to the growing surface. The molecular weight of Poly-D-Lysine is >300,000 Da.

アプリケーション

The level of coating will vary for different species and types of cells. In general final coating concentrations from 3-10μg/cm(2) of surface area will typically supply sufficent coatings for most cell types.



Surface area of plates: (pi)(Radius squared) in cm(2)



In partical terms working dilutions of 50μg/mL to 100μg/mL are usually adequate.



Dilute poly-D-lysine in PBS, water, or 0.1M borate buffer {prepared by adding 1.24 g boric acid and 1.9 g sodium tetraborate in 400 ml water, pH 8.5} to final working concentration.

Plates or flasks are coated for 3 hours to overnight, and up to 3 days if stored at 4C. Best results are usually with just over night coatings.

Remove poly-d-lysine solution and rinse with sterile PBS or water 1-2X. Do not scrape the bottom of the coated plates or flasks.

Use plates immediately or store with PBS for up to 5 days.



Poly-D-lysine coating methods will vary slightly from laboratory to laboratory, please use the method as a general guide.

物理的形状

Liquid. 20ml in sterile water.

保管および安定性

Store at -20C for upto 18 months.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable