AB1530

Sigma-Aldrich

Anti-Peripherin Antibody

serum, Chemicon®

eCl@ss:
32160702
NACRES:
NA.41

由来生物

rabbit

品質水準

100

抗体製品の状態

serum

antibody product type

primary antibodies

クローン

polyclonal

species reactivity

bovine, human, mouse, pig, rat

manufacturer/tradename

Chemicon®

アプリケーション

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

NCBIアクセッション番号

UniProtアクセッション番号

出荷済み

dry ice

詳細

Peripherin is a 56-58 kDa type III intermediate filament protein (Portier et al. 1983), expressed in motor, sensory and sympathetic system neurons as well as neuroendocrine skin carcinomas and certain melanocytic tumors. It is often used an indicator of enteric ganglion cell density in normal and diseased (e.g. Hirschsprung′s disease) states. Abnormal inclusions containing peripherin have also been reported in ALS. During normal, late neurogenesis peripherin appears after the expression of nestin, vimentin, alpha-internexin and neurofilaments and is involved in the differentiation of neurons, namely of the peripheral nervous system, and also probably in axonal regeneration. Peripherin is not related to peripherin-RDS, a photoreceptor protein associated with retinal degeneration and blindness.

特異性

Strong staining on rat and human. Cross-reacts with mouse, pig, and bovine. Other species not tested.
Recognizes Peripherin. The antibody stains a ~57 kDa band cleanly and specifically and does not stain vimentin, GFAP, alpha-internexin or any of the neurofilament subunits. Strong staining on rat, mouse, human, pig and cow peripherin. Does not stain chicken, quail or other more distantly related species which appear to lack peripherin.

免疫原

Electrophoretically pure trp-E-peripherin fusion protein [Dev. Brain Res. (1990) 57:235-248], containing all but the 4 N terminal amino acids of rat peripherin. Fusion protein purified from bacterial inclusion bodies by DEAE-cellulose chromatography in 6 M urea followed by preparative SDS-PAGE.

アプリケーション

Additional Research Applications

Immunohistochemistry:
A previous lot of this antibody was used at 1:100-1:200 dilution. It is suggested that the antibody be used on frozen sections fixed in acetone at -20°C. AB1530 will work on tissue fixed for one hour or less in fresh 4% paraformaldehyde. It has been reported that this antibody can be used on paraffin embedded tissue sections. See Cell & Tissue Research (1997) 288:11-23 & European J. Dermatology (1998) 8:339-342.

Electron Microscopy:
A previous lot of this antibody was used on Electron Microscopy.

Optimal working dilutions and protocols must be determined by end user.
This Anti-Peripherin Antibody is validated for use in IH, IH(P), WB for the detection of Peripherin.
Research Sub Category
感覚・末梢神経

神経細胞及びグリアマーカー
Research Category
ニューロサイエンス

品質

Routinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected peripherin on 10 μg of PC12 lysates.

ターゲットの説明

56-58 kDa

物理的形状

Rabbit polyclonal antisera, no preservatives.
Unpurified

保管および安定性

Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

アナリシスノート

Control
Rat sensory neurons, rat spinal cord homogenate and peripheral nerve homogenate.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

ドイツ水質汚染分類

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable

試験成績書(COA)

原産地証明書(COO)

A L Greenwood et al.
Development (Cambridge, England), 126(16), 3545-3559 (1999-07-20)
Sensory and autonomic neurons of the vertebrate peripheral nervous system are derived from the neural crest. Here we use the expression of lineage-specific transcription factors as a means to identify neuronal subtypes that develop in rat neural crest cultures grown...
A Lysakowski et al.
Hearing research, 133(1-2), 149-154 (1999-07-23)
Recent morphophysiological studies have described three different subpopulations of vestibular afferents. The purpose of this study was to determine whether peripherin, a 56-kDa type III intermediate filament protein present in small sensory neurons in dorsal root ganglion and spiral ganglion...
J M Lay et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 216(2), 190-200 (1999-10-27)
Cholecystokinin (CCK) is a regulatory peptide that is primarily expressed in two adult cell types: endocrine cells of the intestine and neurons of the central nervous system. To determine the ontogeny of CCK expression during intestinal organogenesis, we created a...
Enteroendocrine cell expression of a cholecystokinin gene construct in transgenic mice and cultured cells.
Lay, JM; Bane, G; Brunkan, CS; Davis, J; Lopez-Diaz, L; Samuelson, LC
American Journal of Physiology: Gastrointestinal and Liver Physiology null
S J Morrison et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 20(19), 7370-7376 (2000-09-29)
Isolated neural crest stem cells (NCSCs) differentiate to autonomic neurons in response to bone morphogenetic protein 2 (BMP2) in clonal cultures, but these neurons do not express sympathoadrenal (SA) lineage markers. Whether this reflects a developmental restriction in NCSCs or...

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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