Peripherin is a 56-58 kDa type III intermediate filament protein (Portier et al. 1983), expressed in motor, sensory and sympathetic system neurons as well as neuroendocrine skin carcinomas and certain melanocytic tumors. It is often used an indicator of enteric ganglion cell density in normal and diseased (e.g. Hirschsprung′s disease) states. Abnormal inclusions containing peripherin have also been reported in ALS. During normal, late neurogenesis peripherin appears after the expression of nestin, vimentin, alpha-internexin and neurofilaments and is involved in the differentiation of neurons, namely of the peripheral nervous system, and also probably in axonal regeneration. Peripherin is not related to peripherin-RDS, a photoreceptor protein associated with retinal degeneration and blindness.
Strong staining on rat and human. Cross-reacts with mouse, pig, and bovine. Other species not tested.
Recognizes Peripherin. The antibody stains a ~57 kDa band cleanly and specifically and does not stain vimentin, GFAP, alpha-internexin or any of the neurofilament subunits. Strong staining on rat, mouse, human, pig and cow peripherin. Does not stain chicken, quail or other more distantly related species which appear to lack peripherin.
Electrophoretically pure trp-E-peripherin fusion protein [Dev. Brain Res. (1990) 57:235-248], containing all but the 4 N terminal amino acids of rat peripherin. Fusion protein purified from bacterial inclusion bodies by DEAE-cellulose chromatography in 6 M urea followed by preparative SDS-PAGE.
Additional Research Applications
A previous lot of this antibody was used at 1:100-1:200 dilution. It is suggested that the antibody be used on frozen sections fixed in acetone at -20°C. AB1530 will work on tissue fixed for one hour or less in fresh 4% paraformaldehyde. It has been reported that this antibody can be used on paraffin embedded tissue sections. See Cell & Tissue Research (1997) 288:11-23 & European J. Dermatology (1998) 8:339-342.
A previous lot of this antibody was used on Electron Microscopy.
Optimal working dilutions and protocols must be determined by end user.
This Anti-Peripherin Antibody is validated for use in IH, IH(P), WB for the detection of Peripherin.
Research Sub Category
Routinely evaluated by Western Blot on PC12 lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected peripherin on 10 μg of PC12 lysates.
Rabbit polyclonal antisera, no preservatives.
Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Rat sensory neurons, rat spinal cord homogenate and peripheral nerve homogenate.
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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