AB1534SP

Sigma-Aldrich

Anti-Brain Derived Neurotrophic Factor Antibody

Chemicon®, from rabbit

別名:
BDNF
eCl@ss:
32160702
NACRES:
NA.41

由来生物

rabbit

品質水準

100

antibody product type

primary antibodies

クローン

polyclonal

species reactivity

mouse, rat, human

manufacturer/tradename

Chemicon®

アプリケーション

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

UniProtアクセッション番号

出荷済み

dry ice

Gene Information

human ... BDNF(627)

特異性

Recognizes Brain Derived Neurotrophic Factor (BDNF). By dot blot, less than 0.1% cross-reactivity against mouse NGF, recombinant human NT3 or neurotrophin 4.

免疫原

A 10 amino acid peptide corresponding to the amino-terminal of mouse Brain Derived Neurotrophic Factor (BDNF) coupled to ovalbumin. The sequence is H2N-HSDPARRGEL-COOH.

アプリケーション

Anti-Brain Derived Neurotrophic Factor Antibody is an antibody against Brain Derived Neurotrophic Factor for use in ELISA, IH & WB.
Research Category
ニューロサイエンス
Research Sub Category
神経化学・ニューロトロフィン

神経炎症及び疼痛
Immunohistochemistry: 1-10 μg/mL (see suggested protocol).

Immunoblotting: 1-10 μg/mL. BDNF is not easily extracted in standard buffers. Dissected tissues should be homogenized in 10 volumes of 100mM phosphate buffer containing 1mM EDTA, 2M guanidine hydrochloride (pH7.2), and three protease inhibitors, 10mM N-ethylmaleimide, 0.36mM pepstatin, and 1mM PMSF. Homogenates are then sonicated and centrifuged at 46K x g for 30 minutes at 4°C. BDNF migrates as 18 kDa, with a homodimer at ~27-30 kDa.

ELISA (one-site): 1-10 μg/mL

Optimal working dilutions must be determined by end user.

ターゲットの説明

18 kDa (monomer) and 27-30 kDa (homodimer)

物理的形状

Format: Purified
Protein A Purified immunoglobulin fraction lyophilized from PBS, with no preservatives. Reconstitute with 500 μL of sterile distilled water (1 mg/mL).
Protein A Purfied

保管および安定性

Maintain at -20 to -70°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.

アナリシスノート

Control
Brain tissue

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

ドイツ水質汚染分類

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable

Comparison of the influence of two models of mild stress on hippocampal brain-derived neurotrophin factor (BDNF) immunoreactivity in old age rats.
Badowska-Szalewska, et al.
Acta Neurobiologiae Experimentalis, 77, 68-76 (2019)
Antonino Germanà et al.
International journal of molecular sciences, 21(16) (2020-08-19)
Brain-derived neurotrophic factor (BDNF), a member of the neurotrophin family, is involved in multiple and fundamental functions of the central and peripheral nervous systems including sensory organs. Despite recent advances in knowledge on the functional significance of BDNF and TrkB...
Luciano Domenici et al.
PloS one, 9(12), e115579-e115579 (2014-12-24)
Vision loss in glaucoma is caused by progressive dysfunction of retinal ganglion cells (RGCs) and optic nerve atrophy. Here, we investigated the effectiveness of BDNF treatment to preserve vision in a glaucoma experimental model. As an established experimental model, we...
Differential expression of neurotrophins in postnatal C57BL/6 mice striatum.
V Zerme?o,S Espindola,E Mendoza,E Hernandez-Echeagaray
International Journal of Biological Sciences null
A Germanà et al.
Journal of anatomy, 217(3), 214-222 (2010-07-24)
Brain-derived neurotrophic factor (BDNF) signaling through TrkB regulates different aspects of neuronal development, including survival, axonal and dendritic growth, and synapse formation. Despite recent advances in our understanding of the functional significance of BDNF and TrkB in the retina, the...

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