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由来生物
sheep
品質水準
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
primary antibodies
クローン
polyclonal
精製方法
affinity chromatography
化学種の反応性
human
テクニック
ChIP: suitable
western blot: suitable
アイソタイプ
IgG
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... POLR2M(81488)
詳細
Gdown1 (DNA-directed RNA polymerase II subunit GRINL1A; DNA-directed RNA polymerase II subunit M; glutamate receptor-like protein 1A) is a nuclear protein belonging to the GRINL1 family. It binds to the RNA Polymerase II (G) molecule (RNAPII (G)) and prevents its interaction with transactivating factors, thereby suppressing RNAPII (G)-mediated transcriptional events. Gdown1 also indirectly suppresses RNAPII (G)-transcription by blocking the binding of the elongation-enhancing factor TFIIF to the elongation complex. Gdown1 also prevents TFIIF-mediated assembly of the preinitiation complex required for recruiting RNAPII to the promoter region, by binding competitively to Pol II at the RPB1 and RPB5 subunits recognized by TFIIF. Gdown1 may also inhibit the termination factor TTF2. The effects of Gdown1 are blocked by the P-TEFb. Overall, Gdown1 regulates the transcriptional activity of RNAPII (G) at multiple promoter regions by various mechanisms. Gdown1 has been detected in brain, testis, prostate, lung, small intestine, heart, kidney, and skeletal muscle.
免疫原
Histidine-tagged recombinant protein corresponding to human Gdown1.
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
RNA代謝及び結合タンパク質
RNA代謝及び結合タンパク質
Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in HeLa cells. (Price, D., et al. (2012). Cell. 45(1):38-50.)
Chromatin Immunoprecipitation Sequencing Analysis: A representative lot was used by an independent laboratory in HeLa cells. (Price, D., et al. (2012). Cell. 45(1):38-50.)
Chromatin Immunoprecipitation Sequencing Analysis: A representative lot was used by an independent laboratory in HeLa cells. (Price, D., et al. (2012). Cell. 45(1):38-50.)
Use Anti-Gdown1 Antibody (Sheep Polyclonal Antibody) validated in WB, ChIP to detect Gdown1 also known as Myocardial zonula adherens protein, GRINL1A upstream protein, Gup.
品質
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Gdown1 on 10 µg of HeLa cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Gdown1 on 10 µg of HeLa cell lysate.
ターゲットの説明
~50 kDa observed. Uniprot describes 11 isoforms ranging between 44 and 64 kDa
物理的形状
Affinity purified
Purified sheep polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
保管および安定性
Stable for 1 year at 2-8°C from date of receipt.
アナリシスノート
Control
HeLa cell lysate
HeLa cell lysate
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
ABE23:
試験成績書（COA）
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