Specific for human glucose transporter-4 from muscle and adipose tissue. The antisera does not cross-react with GLUT-1. The molecular weight of the glucose transporter precipitated by the serum is approximately 46 kDa.
免疫原
Partially purified GLUT-4
アプリケーション
Research Category 細胞シグナル伝達
Research Sub Category インスリン/エネルギーシグナル伝達
This Anti-Glucose Transporter GLUT-4 Antibody is validated for use in IP, WB for the detection of Glucose Transporter GLUT-4.
Western blot analysis of GLUT-4 activity in adipose and muscle tissue (5μg/ml)
Immunoprecipitation of GLUT-4 (5μg/ml)
Optimal working dilutions must be determined by the end user.
ターゲットの説明
46 kDa
物理的形状
ImmunoAffinity Purified
Purified immunoglobulin in PBS with 0.01% sodium azide.
保管および安定性
For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
アナリシスノート
Control Positive Control: 3T3 L1 adipocyte membranes
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
American journal of physiology. Regulatory, integrative and comparative physiology, 303(10), R1062-R1070 (2012-10-12)
High-fat (HF) diets impair skeletal muscle response to the insulin-sensitizing adipokine adiponectin (Ad) in rodents, preceding the development of insulin resistance. Skeletal muscle insulin response in HF-fed rats can be restored with chronic exercise; whether recovery of skeletal muscle Ad
Previous studies demonstrated that acute exercise can enhance glucose uptake (GU), γ3-AMP-activated protein kinase (AMPK) activity, and Akt substrate of 160 kDa (AS160) phosphorylation in skeletal muscles from low-fat diet (LFD)- and high-fat diet (HFD)-fed male rats. Because little is
Attenuated skeletal muscle glucose uptake (GU) has been observed with advancing age. It is important to elucidate the mechanisms linked to interventions that oppose this detrimental outcome. Earlier research using young rodents and (or) cultured myocytes reported that treatment with
The Rab GTPase activating protein known as Akt substrate of 160 kDa (AS160 or TBC1D4) regulates insulin-stimulated glucose uptake in skeletal muscle, the heart, and white adipose tissue (WAT). A novel rat AS160-knockout (AS160-KO) was created with CRISPR/Cas9 technology. Because
Biochimica et biophysica acta, 1822(11), 1735-1740 (2012-08-01)
Calorie restriction (CR; ~60% of ad libitum, AL, consumption) improves insulin-stimulated glucose uptake in skeletal muscle. The precise cellular mechanism for this healthful outcome is unknown, but it is accompanied by enhanced insulin-stimulated activation of Akt. Previous research using Akt2-null