Antibody MAB13439 is directed against a synthetic peptide corresponding to the amino terminal region of human MMP-1. The antibody binds to MMP-1 but does not cross-react with the other MMP family members (MMP-2, MMP-3, MMP-9, etc.).
Reacts with human. Other species not tested; however this sequence of MMP-1 is well conserved across species. Higher concentrations of antibody may be needed for non-human samples.
Western blot: 1:1000 when using colorimetric substrates; 1:5,000 for chemiluminescent substrates. When used against the reduced protein, antibody MAB13439 recognizes bands at 53 kDa and 51 kDa (the pro-form), as well as active forms.
Collagenase levels in quiescent cells and tissues is minimal, and stimulation or protein concentration is often needed to visualize the bands. In addition, cell types differ greatly in the quantity of collagenase produced.
Optimal working dilutions must be determined by end user.
This Anti-MMP-1 Antibody, clone 4E7.2 is validated for use in ELISA, WB for the detection of MMP-1.
Research Sub Category
Purified immunoglobulin. Liquid at 1 mg/mL containing 0.1% sodium azide.
Maintain at -20°C in undiluted aliquots for up to 12 months.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.