MAB5280

Sigma-Aldrich

Anti-Tyrosine Hydroxylase Antibody, clone 2/40/15

clone 2/40/15, Chemicon®, from mouse

別名:
TH, Tyrosine Monooxygenase
eCl@ss:
32160702
NACRES:
NA.41

品質水準

100

由来生物

mouse

抗体製品の状態

purified immunoglobulin

antibody product type

primary antibodies

クローン

2/40/15, monoclonal

species reactivity

chicken, quail, rat, bovine

manufacturer/tradename

Chemicon®

アプリケーション

immunohistochemistry: suitable
western blot: suitable

アイソタイプ

IgG2a

NCBIアクセッション番号

UniProtアクセッション番号

出荷済み

wet ice

詳細

Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.

特異性

Tyrosine hydroxylase (TH) catalyzes the rate-determining initial step in the biosynthesis of catecholamines such as dopamine, noradrenaline and adrenaline. Therefore the antibody is used to stain dopaminergeous and adrenergeous neurons as well as the chromaffine tissue of the adrenal medulla. Cell lines such as the PC 12 line, which is derived from a rat pheochromocytoma also, show positive reactions. In immunoblots of adrenal medulla tissue, the antibody recognizes a single protein band in the molecular weight range of 56 - 60 kD.

免疫原

Purified tyrosine hydroxylase (EC 1.14.16.2) from a rat pheochromocytoma (2).

アプリケーション

Immunohistochemistry:
1:100-1:500. A previous lot of this antibody was used in immunohistochemistry.

Western blot:
1:1000-1:2000

Optimal working dilutions must be determined by end user.
Research Sub Category
神経伝達物質及び受容体

神経細胞及びグリアマーカー
Research Category
ニューロサイエンス
This Anti-Tyrosine Hydroxylase Antibody, clone 2/40/15 is validated for use in IH, WB for the detection of Tyrosine Hydroxylase.

品質

Routinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected Tyrosine Hydroxylase on 10 μg of PC12 lysates.

ターゲットの説明

56-60 kDa

物理的形状

Purified mouse monoclonal IgG2a in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.
Format: Purified
Protein A purified

保管および安定性

Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.

アナリシスノート

Control
Positive Control: Brain (sympathetic nerve terminals) and adrenal gland tissue

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

10 - Combustible liquids

ドイツ水質汚染分類

WGK 2

試験成績書(COA)

原産地証明書(COO)

Regulation of natural cell death in dopaminergic neurons of the substantia nigra by striatal glial cell line-derived neurotrophic factor in vivo.
Tinmarla Frances Oo, Nikolai Kholodilov, Robert E Burke
The Journal of Neuroscience null
Mapping of reinforcing and analgesic effects of the mu opioid agonist Endomorphin-1 in the ventral midbrain of the rat.
Thomas C Jhou,Sheng-Ping Xu,Mary R Lee,Courtney L Gallen,Satoshi Ikemoto
Psychopharmacology (Psychopharmacologia) null
Differential effects of l-DOPA on monoamine metabolism, cell survival and glutathione production in midbrain neuronal-enriched cultures from parkin knockout and wild-type mice
Casarejos, M J, et al
Journal of Neurochemistry, 94, 1005-1014 (2005)
Javier Blesa et al.
Frontiers in systems neuroscience, 5, 92-92 (2012-01-31)
The onset of Parkinson's disease (PD) is characterized by focal motor features in one body part, which are usually correlated with greater dopaminergic depletion in the contralateral posterior putamen. The role of dopamine (DA) hemispheric differences in the onset and...
A Chocyk et al.
Neuroscience, 182, 43-61 (2011-03-15)
Early life stressors have life-long functional and anatomical consequences. Though many neurotransmitters are involved in the functional impact of early life stress, dopamine seems to be important because of its roles in motor control, adaptation to stressful conditions, mood, cognition...

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

テクニカルサービス連絡先