Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
Tyrosine hydroxylase (TH) catalyzes the rate-determining initial step in the biosynthesis of catecholamines such as dopamine, noradrenaline and adrenaline. Therefore the antibody is used to stain dopaminergeous and adrenergeous neurons as well as the chromaffine tissue of the adrenal medulla. Cell lines such as the PC 12 line, which is derived from a rat pheochromocytoma also, show positive reactions. In immunoblots of adrenal medulla tissue, the antibody recognizes a single protein band in the molecular weight range of 56 - 60 kD.
Purified tyrosine hydroxylase (EC 22.214.171.124) from a rat pheochromocytoma (2).
1:100-1:500. A previous lot of this antibody was used in immunohistochemistry.
Optimal working dilutions must be determined by end user.
Research Sub Category
This Anti-Tyrosine Hydroxylase Antibody, clone 2/40/15 is validated for use in IH, WB for the detection of Tyrosine Hydroxylase.
Routinely evaluated by Western Blot on PC12 lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected Tyrosine Hydroxylase on 10 μg of PC12 lysates.
Purified mouse monoclonal IgG2a in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.
Protein A purified
Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.
Positive Control: Brain (sympathetic nerve terminals) and adrenal gland tissue
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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