Cyclic AMP-responsive element-binding protein 3-like protein 1 (UniProt Q9Z125; also known as BBF-2 homolog, cAMP-responsive element-binding protein 3-like protein 1, Old astrocyte specifically-induced substance) is encoded by the Creb3l1 (also known as Oasis) gene (Gene ID 26427) in murine species. Creb3l1 belongs to the the old astrocyte specifically induced substance (OASIS) family of transcription factors that function as ER stress transducers. OASIS members possess both a transcription-activation domain and a basic leucine zipper (bZIP) domain, as well as a transmembrane domain that allows them to associate with the ER. OASIS family members, except Luman (Creb3), exhibit cell- and tissue-specific expression patterns, with Oasis (Creb3l1) being preferentially expressed in osteoblasts and astrocytes, AIbZIP (Creb3l4, Creb4, Tisp40) in testis and prostate, Bbf2h7 (Creb3l2) in chondrocytes, and Crebh (Creb3l3) in hepatocytes. OASIS family members are activated in response to ER stress via a distinct mechanism than the ER unfold protein response (UPR) transducer ATF6. Oasis (Creb3l1) and Bbf2h7 (Creb3l2) are constantly ubiquitinated by the ER-resident E3 ubiquitin ligase HMG-CoA reductase degradation 1 (Hrd1) and degraded by proteasome under normal conditions. ER stress stabilizes Oasis and Bbf2h7 by inducing a dissociation of these transcription factors from Hrd1.
Expected to react with both spliced isoforms as well as the N-terminal fragment of cleaved Oasis.
Flag-tagged recombinant protein corresponding to the N-terminal half of mouse OASIS/CREB3L1.
Epitope: N-terminal half
Research Sub Category
This Anti-OASIS/CREB3L1 Antibody, clone 44C7 is validated for use in Western Blotting for the detection of OASIS/CREB3L1.
Western Blotting Analysis: A representative lot detected full-length OASIS in lysates from untreated MC3T3-E1 murine osteoblastic cells and cleaved N-terminal OASIS fragment in lysates from MC3T3-E1 cells subjected to ER stresser thapsigargin or brefeldin A treatment (Murakami, T., et al. (2009). Nat. Cell Biol. 11(10):1205-1211).
Western Blotting Analysis: A representative lot detected a higher OASIS expression level in Hrd1-/- than in wild-type MEFs. A more elevated OASIS level was also detected in osteoblasts differentiated from Hrd1-/- than from wild-type MEFs following bone morphogenetic protein 2 (BMP2) treatment (Kondo, S., et al. (2012). Cell Death Differ. 19(12):1939-1949).
Evaluated by Western Blotting in MC3T3-E1 mouse osteoblastic cell lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected OASIS/CREB3L1 in 10 µg of MC3T3-E1 mouse osteoblastic cell lysate.
~62 and 52 kDa observed
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
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