Merck
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MABE343-AF647

Sigma-Aldrich

Anti-Puromycin, clone 12D10, Alexa Fluor® 647 Conjugate Antibody

clone 12D10, 0.5 mg/mL, from mouse

eCl@ss:
32160702
NACRES:
NA.41

由来生物

mouse

品質水準

100

結合体

ALEXA FLUOR® 647

抗体製品の状態

purified antibody

antibody product type

primary antibodies

クローン

12D10, monoclonal

species reactivity

human

濃度

0.5 mg/mL

technique(s)

immunocytochemistry: suitable

輸送温度

wet ice

詳細

Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3′ end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
This Anti-Puromycin, clone 12D10 is validated for use in Immunocytochemistry for the detection of Puromycin.

特異性

Predicted to react with cells from all species when pre-incubated with puromycin.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子
Research Sub Category
細胞周期、DNA複製及び修復

品質

Evaluated by Immunocytochemistry Analysis untreated and puromycin treated HeLa cells.

Immunocytochemistry Analysis: A 1:2,500 dilution of this antibody detected puromycin-incorporated neosynthesized proteins in puromycin treated HeLa cells.

Alexa Fluor® is a registered trademark of Life Technologies.

ターゲットの説明

Refer to Cat. No. MABE343 for observed molecular weight information.

物理的形状

Protein G Purified

法的情報

ALEXA FLUOR is a registered trademark of Life Technologies

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

保管分類コード

12 - Non Combustible Liquids

WGK

WGK 2

引火点(°F)

Not applicable

引火点(℃)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

非該当

ISHL Notified Names

非該当

Certificate of Analysis

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Certificate of Quality

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