Merck
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MABF121

Sigma-Aldrich

Anti-SV40 Large T Antigen Antibody, clone PAb416

clone PAb416, from mouse

Synonym(s):
Large T antigen, LT, LT-AG
eCl@ss:
32160702
NACRES:
NA.41

由来生物

mouse

品質水準

100

抗体製品の状態

purified antibody

antibody product type

primary antibodies

クローン

PAb416, monoclonal

species reactivity

SV40-infected cells

technique(s)

immunocytochemistry: suitable
western blot: suitable

アイソタイプ

IgG2aκ

NCBIアクセッション番号

UniProtアクセッション番号

輸送温度

wet ice

詳細

Both the Simian virus (SV40) large T and small T antigens are encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the P105 product of the retinoblastoma susceptibility gene.

特異性

This antibody recognizes the large T antigen of SV40.

免疫原

Epitope: Large T antigen of SV40
Purified large T-antigen of SV40

アプリケーション

Research Category
炎症及び免疫
Research Sub Category
免疫グロブリン及び免疫
Anti-SV40 Large T Antigen Antibody, clone PAb416 is an antibody against SV40 Large T Antigen for use in Western Blotting, ICC.
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected SV40 Large T Antigen in ICC (Sabatier, J., et al. (2005). 58(4):429-431.; Del Valle, L., et al. (2004). J Virol. 78(7):3462-3469.).

品質

Evaluated by Western Blot in Cos-1 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected SV40 Large T Antigen in 10 µg of Cos-1 cell lysate.

ターゲットの説明

~82 kDa observed

物理的形状

Protein G Purified
Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
Cos-1 cell lysate

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

保管分類コード

12 - Non Combustible Liquids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable

Certificate of Analysis

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Certificate of Quality

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