Merck
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MABT523

Sigma-Aldrich

Anti-beta-Actin Antibody, clone RM112

clone RM112, from rabbit

Synonym(s):
Actin, cytoplasmic 1, Beta-actin, beta-Actin
eCl@ss:
32160702
NACRES:
NA.41

由来生物

rabbit

品質水準

100

抗体製品の状態

purified antibody

antibody product type

primary antibodies

クローン

RM112, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable
western blot: suitable

アイソタイプ

IgG

UniProtアクセッション番号

輸送温度

wet ice

Gene Information

human ... ACTB(60)

詳細

Actin is a 43 kDa highly conserved structural protein. There are three main actin isotypes (alpha, beta and gamma) which show >90% amino-acid (aa) homology between isotypes and >98% homology within members of a particular isotypic group. The majority of the isotype heterogeneity is located in the amino-terminal 30 aa. The different actin isotypes have been shown to behave very differently in vitro and in vivo. Recent studies describe differential subcellular localization of gamma-actin and isotype specific binding of actin associated proteins. Beta actin is one of the two nonmuscle cytoskeletal actins and is relatively stable and appears to remain at normal levels regardless of experimental treatment. It is generally used as an internal control for experiments.

免疫原

A peptide corresponding to the N-terminus of beta-Actin.

アプリケーション

Research Category
細胞骨格
Research Sub Category
接着分子(CAM)
Immunocytochemistry Analysis (ICC): A 1:200 dilution from a representative lot detected beta-Actin in A431 cells.

This Anti-beta-Actin Antibody, clone RM112 is validated for use in Western Blotting, Immunocytochemistry for the detection of beta-Actin.

品質

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected beta-Actin in A431 cell lysate.

ターゲットの説明

~45 kDa observed. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Protein A purified
Format: Purified

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificate of Analysis

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Certificate of Quality

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