These tubes should not be autoclaved prior to use, as we have not studied this for integrity. Tubes can be sanitized with 70% ethanol. However, the ethanol only sanitizes the device; it does not sterilize it.
UFC5010
Amicon® Ultra Centrifugal Filter, 10 kDa MWCO
sample volume 0.5 mL, regenerated cellulose membrane, MWCO 10 kDa
Amicon® Ultra Centrifugal Filter, 10 kDa MWCO
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別名:
Centrifugal concentrator, 10 kDa MWCO, 0.5 mL sample volume
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この商品について
UNSPSC Code:
41104916
NACRES:
NB.24
eCl@ss:
36100101
Material:
polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing
Pore size:
10 kDa MWCO
Working volume:
≤500 μL , 0.5 mL
Sterility:
non-sterile
Packaging:
pkg of 24 pkg, pkg of 500 pkg, pkg of 8 pkg, pkg of 96 pkg
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material
polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing
sterility
non-sterile
product line
Amicon®
packaging
pkg of 24 pkg, pkg of 500 pkg, pkg of 8 pkg, pkg of 96 pkg
manufacturer/tradename
Amicon® Ultra
sustainability
Greener Alternative Product
parameter
0.5 mL sample volume
technique(s)
protein extraction: suitable, protein purification: suitable
L
4.99 cm (1.96 in.)
diam.
10.8 mm
filtration area
1 cm2
volume
0.5 mL
working volume
≤500 μL , 0.5 mL
pore size
10 kDa MWCO
Quality Level
greener alternative category
shipped in
ambient
1 of 4
当該品目 | UFC9010 | UFC8010 | UFC201024 |
|---|---|---|---|
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra |
| pore size 10 kDa MWCO | pore size 10 kDa MWCO | pore size 10 kDa MWCO | pore size 10 kDa MWCO |
| material polypropylene cap, regenerated cellulose membrane, polypropylene tube, styrene-butadiene housing | material polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing | material polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing | material polypropylene tube (for collection), regenerated cellulose membrane, styrene-butadiene housing |
| parameter 0.5 mL sample volume | parameter 15 mL sample volume | parameter 4 mL sample volume | parameter 2 mL sample volume |
| product line Amicon® | product line Amicon® | product line Amicon® | product line Amicon® |
General description
Amicon® Ultra-0.5 centrifugal filter devices provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, Cytochrome c (12.4 kDa) at 0.25 mg/ml initial concentration is typically retained at >95% .
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
Amicon® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 0.5 mL filters deliver great performance and lower spin times.
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
Amicon® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 0.5 mL filters deliver great performance and lower spin times.
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from bio-circular material allocated using the ISCC mass balance approach. Click here for more information.
Application
- Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel® membrane
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
- Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC
- Desalting, buffer exchange, and protein dialysis
Features and Benefits
- Ultracel® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90%
- Concentrates 500 μL down to 15 μL
- Concentration factor 25x to 30x
- High sample recovery (greater than 90% of dilute starting solution)
- Fast processing time: 10 to 30 minutes
- Reverse spin capability provides consistent recoveries
Physical form
Color Code: Clear
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
資料
ナノ粒子の精製と製造における、ろ過法とメンブレンフィルターの選定。
溶媒交換やサンプル濃縮などのタンパク質サンプル調製のための限外ろ過膜の原理と利点をご覧ください。
Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.
PNGase Fast denaturing buffer and enzyme provide results similar to a conventional 20-hour protocol, reducing workflow time to about 1 hour.
プロトコル
Detergents are frequently used to solubilize proteins and nucleic acids during purification, but the presence of detergents may interfere with downstream analyses.
Best practices for standardizing and reducing the Extracellular Vesicle Preparation workflow, and eliminating contamination of EV preparations with dead-cell-derived vesicles.
If an antibody must be purified from a small sample volume (0.5-10 mL), then the Amicon® Pro Purification System is recommended.
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Can the polypropylene collection tubes be autoclaved prior to the filtration? And is there a way to sterilize the filter e.g. with 70% ethanol?
1 回答-
役に立ちましたか？
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Can I purchase just the flow through columns?
1 回答-
The Amicon Ultra products come in either 2 piece or 3 piece configurations. It is not possible to order just the filter inner tube or the empty outer tube. These two pieces fit together and are sold together as they are always needed. One sample, one centrifugal product. With the Amicon Ultra 0.5 or 2, we have a third piece, an outer collection tube added which is optionally used for reverse spin capability. Please consult our User Guide.
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What is the minimum volume allowed by the device? If we have a volume of 50 microliters, is it advisable to dilute it in PBS to obtain a larger volume, closer to 500 microliters?
1 回答-
Diluting to 500 ul is optional. You have to consider what level of concentration is expected. Amicons can concentrate down to about 15 ul, so a 50 ul sample can only be concentrated ~3.3 fold.
役に立ちましたか？
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What is the pore diameter of Amicon® Ultra Centrifugal Filter, 10 kDa
1 回答-
The ultrafiltration membrane in this product is characterized by a nominal molecular weight limit based on protein retention. An absolute linear pore diameter is not determined. However, a membrane with a 10 kDa molecular weight cutoff will retain nanoparticles of 3-9 nanometers. Please see the related article on nanoparticle ultrafiltration: https://www.sigmaaldrich.com/technical-documents/technical-article/analytical-chemistry/filtration/nanoparticle-ultrafiltration
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What is the tolerance for molecular weight with these spin columns? Could you reasonably purify a protein of 15 kDa using these columns, or are the tolerances too high and would result in loss of protein into the filtrate?
1 回答-
It is recommended to choose a molecular weight cut-off that is at least two times smaller than the molecular weight of the target molecules.
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Is this filter suitable for purifying MHC-bound peptides as filtrate/eluate while trapping proteins and antibodies as retentate following Immunoprecipitation?
1 回答-
This product is not suitable for separating protein/peptides of different sizes.
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If a centrifuge is unable to achieve a spinning speed of 14000 X g as recommended in the protocol, is it possible to compensate by spinning the samples for a longer duration?
1 回答-
Yes, it is possible to compensate for a lower centrifuge spinning speed by increasing the duration. This can be achieved by calculating the K factor, which determines the adjustment needed in time.
役に立ちましたか？
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se puede utilizar los UFC501024 para concentrar una proteina glicosilada?
1 回答-
Yes, Amicon devices like UFC501024 can be used to concentrate any protein greater than about 20 kDa.
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Dear Sir/Mam, can you let me know how many tubes is 8 pkg? Is it 8 tubes? Best regards, Alejandra
1 回答-
It is 8 tubes per pack.
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What is the maximum RPM that this can be spun to ensure the membrane does not perforate?
1 回答-
The recommended G force for the Amicon® Ultra-0.5 filter is 14,000 g & 1,000 g reverse spin when using a Fixed-Angle (35 º) Rotor.
Please refer to the brochure for more information: details: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/260/181/br5002en-protein-prep-workflow-ms.pdf
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