The Amicon Ultra devices are non-sterile. However, many customers rinse with 70% IPA then discard, let dry, then introduce a sample to the product.
UFC8003
Amicon® Ultra Centrifugal Filter, 3 kDa MWCO
sample volume 4 mL, regenerated cellulose membrane, MWCO 3 kDa
別名:
Centrifugal concentrator, 3 kDa MWCO, 4 mL sample volume
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| 容量 | カタログ番号 | 在庫状況 | 単価 |
|---|
この商品について
UNSPSC Code:
41104916
NACRES:
NB.24
eCl@ss:
36100101
Material:
polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing
Pore size:
3 kDa MWCO
Working volume:
≤4 mL , 4 mL
Sterility:
non-sterile
Packaging:
pkg of 24 pkg, pkg of 8 pkg, pkg of 96 pkg
この製品は、Merck Milliporeウェブサイトでのみ注文できます。
Quality Level
material
polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing
sterility
non-sterile
product line
Amicon®
packaging
pkg of 24 pkg, pkg of 8 pkg, pkg of 96 pkg
manufacturer/tradename
Amicon® Ultra
sustainability
Greener Alternative Product
parameter
4 mL sample volume
technique(s)
protein extraction: suitable, protein purification: suitable
L
124 mm (4.88 in.)
diam.
17.3 mm
filtration area
3 cm2
volume
4 mL
working volume
≤4 mL , 4 mL
pore size
3 kDa MWCO
greener alternative category
shipped in
ambient
General description
Amicon® Ultra 4 mL centrifugal devices with Ultracel® 3,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, Cytochrome c (12.4 kDa) at 0.25 mg/ml initial concentration is retained at >95% .
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from 60% bio-circular material allocated using the ISCC mass balance approach. Click here for more information.
Application
- Ultrafiltration with 3,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC.
- Concentration of dilute or pre-purified proteins from column eluents.
- Desalting, buffer exchange and protein dialysis.
- Size exclusion and protein fractionation.
Features and Benefits
- Ultracel® regenerated cellulose membrane with 3 kDa NMWCO delivers retentate recovery of greater than 90%.
- Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes).
- Heat-sealed membrane minimizes downstream extractables.
- 100% integrity tested for reliable performance.
- Convenient sample monitoring with translucent housing and volume gradations.
- Direct pipette sample access eliminates processing step to recover concentrate.
- High concentration factors of 80–100X.
Physical form
Color Code: Clear with pink cap
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
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当該品目 | |||
|---|---|---|---|
| parameter 4 mL sample volume | parameter 2 mL sample volume | parameter 15 mL sample volume | parameter 0.5 mL sample volume |
| pore size 3 kDa MWCO | pore size 3 kDa MWCO | pore size 3 kDa MWCO | pore size 3 kDa MWCO |
| manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| material polyethylene cap, polypropylene tube, regenerated cellulose support, polyethylene liner, styrene-butadiene housing, regenerated cellulose membrane | material polypropylene tube (for collection), regenerated cellulose membrane, styrene-butadiene housing | material polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing | material polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing |
| working volume ≤4 mL | working volume ≤2 mL | working volume ≤15 mL | working volume ≤500 μL , 0.5 mL |
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
プロトコル
ヒト血漿プロテオームは、バイオマーカーの発見と検討を行うための豊富な資源です。
The human plasma proteome is a vast resource for biomarker discovery and investigation.
資料
ナノ粒子の精製と製造における、ろ過法とメンブレンフィルターの選定。
溶媒交換やサンプル濃縮などのタンパク質サンプル調製のための限外ろ過膜の原理と利点をご覧ください。
Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| UFC800396 | 08436037126024 |
| UFC800324 | 08436037126017 |
| UFC800308 | 04053252265938 |
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Good day, I would like to know if these concentrators are sterile and the recovered proteins are suitable for downstream cell culture assays. Thank you!
1 回答-
役に立ちましたか？
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What could be the reason for no protein recovery (in the concentrate or flow through) while using the UFC800324 to purify protein? Could the protein may be absorbed in the filter?
1 回答-
The low or no protein recovery issue may be attributed to over-concentration of the protein, leading to precipitation. The User Guide provides guidance on maximizing sample recovery: Low sample recovery in the concentrate may be due to adsorptive losses, over-concentration, or passage of sample through the membrane. Adsorptive losses depend upon solute concentration, its hydrophobic nature, temperature and time of contact with filter device surfaces, sample composition, and pH. To minimize losses, remove concentrated samples immediately after centrifugal spin. If the starting sample concentration is high, monitor the centrifugation process in order to avoid over-concentration of the sample. Over-concentration can lead to precipitation and potential sample loss. If the sample appears to be passing through the membrane, choose a lower MWCO Amicon device.
役に立ちましたか？
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What is max g force these filters can take? i.e. what sort of rpm or g force should I spin at?
1 回答-
The UFC8003 protocol states to spin at 7,500 × g maximum for approximately 10–40 minutes. See the image below for further details.
役に立ちましたか？
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Can we centrifuge these tubes in a normal 15 mL swinging bucket centrifuge?
1 回答-
Yes, the device can be used in centrifuges with a swinging bucket or fixed-angle rotor.
役に立ちましたか？
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