The Amicon Ultra 0.5 and 2 have reverse spin capability. The Amicon Ultra 4 and 15 do not.
UFC8100
Amicon® Ultra Centrifugal Filter, 100 kDa MWCO
sample volume 4 mL, regenerated cellulose membrane, MWCO 100 kDa
Amicon® Ultra Centrifugal Filter, 100 kDa MWCO
別名:
Centrifugal concentrator,100 kDa MWCO, 4 mL sample volume
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| 容量 | カタログ番号 | 在庫状況 | 単価 |
|---|
この商品について
UNSPSC Code:
41104916
NACRES:
NB.24
eCl@ss:
36100101
Material:
polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing
Pore size:
100 kDa MWCO
Working volume:
≤4 mL , 4 mL
Sterility:
non-sterile
Packaging:
pkg of 24 pkg, pkg of 8 pkg, pkg of 96 pkg
この製品は、Merck Milliporeウェブサイトでのみ注文できます。
Quality Level
material
polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing
sterility
non-sterile
product line
Amicon®
packaging
pkg of 24 pkg, pkg of 8 pkg, pkg of 96 pkg
manufacturer/tradename
Amicon® Ultra
sustainability
Greener Alternative Product
parameter
4 mL sample volume
technique(s)
protein extraction: suitable, protein purification: suitable
L
124 mm (4.88 in.)
diam.
17.3 mm
filtration area
3 cm2
volume
4 mL
working volume
≤4 mL , 4 mL
pore size
100 kDa MWCO
greener alternative category
shipped in
ambient
General description
Amicon® Ultra 4 mL centrifugal devices with Ultracel® 100,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, IgG (156 kDa) at 1 mg/ml initial concentration is retained at >95% .
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from 60% bio-circular material allocated using the ISCC mass balance approach. Click here for more information.
Application
- Ultrafiltration with 100,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC.
- Concentration of dilute or pre-purified proteins from column eluents.
- Desalting, buffer exchange and protein dialysis.
- Size exclusion and protein fractionation.
Features and Benefits
- Ultracel® regenerated cellulose membrane with 100 kDa NMWCO delivers retentate recovery of greater than 90%.
- Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes).
- Heat-sealed membrane minimizes downstream extractables.
- 100% integrity tested for reliable performance.
- Convenient sample monitoring with translucent housing and volume gradations.
- Direct pipette sample access eliminates processing step to recover concentrate.
- High concentration factors of 80–100X.
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
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当該品目 | |||
|---|---|---|---|
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| pore size 100 kDa MWCO | pore size 100 kDa MWCO | pore size 100 kDa MWCO | pore size 100 kDa MWCO |
| manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra |
| parameter 4 mL sample volume | parameter 2 mL sample volume | parameter 0.5 mL sample volume | parameter 15 mL sample volume |
| material polyethylene cap, polypropylene tube, regenerated cellulose support, polyethylene liner, styrene-butadiene housing, regenerated cellulose membrane | material polypropylene tube (for collection), regenerated cellulose membrane, styrene-butadiene housing | material polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing | material polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing |
| working volume ≤4 mL | working volume ≤2 mL | working volume ≤500 μL , 0.5 mL | working volume ≤15 mL |
保管分類
11 - Combustible Solids
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
プロトコル
The human plasma proteome is a vast resource for biomarker discovery and investigation.
ヒト血漿プロテオームは、バイオマーカーの発見と検討を行うための豊富な資源です。
資料
Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.
Filtration methods and membrane filter selection in nanoparticle purification and production.
ナノ粒子の精製と製造における、ろ過法とメンブレンフィルターの選定。
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| UFC810008 | 04053252612169 |
| UFC810024 | 04053252666377 |
| UFC810096 | 04053252726576 |
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Are these units able to perform a reverse spin to collect contents, or do contents need to be collected via pipet?
1 回答-
役に立ちましたか？
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What is the maximum recommended starting protein concentration from Vero cell lysate to avoid overwhelming the filter?
1 回答-
There is no maximum protein load for these devices, and optimal protein concentrations must be determined empirically. We do recommend a clarifying spin prior to filtration to bring down larger particulates.
役に立ちましたか？
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Can I use Amicon Ultra 100 kDa filter only for protein sample filtration, to avoid concentration or sample purification?
1 回答-
This device is intended for use as a protein concentrator. Proteins above 100 kDa will be retained while smaller proteins will pass through to the filtrate fraction. Other particles within the sample may be present in both the filtrate and the retentate, depending on the nature of the particle, the nature of the filter membrane, and the centrifugation protocol or technique used. To discuss this further, we kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice and click on "Product Technical Inquires" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.
役に立ちましたか？
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Les Amicons sont-il RNase/DNase free- DNA/RNA free? Traitement à l'oxyde d'éthylène?
1 回答-
The Amicon products are not tested for RNase/DNase activity. These items are not considered RNase/DNase free. They are not ethylene oxide treated.
役に立ちましたか？
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