Merck
All Photos(1)

60983

Millipore

Kovacインドール試薬

suitable for microbiology

Synonym(s):
4-(ジメチルアミノ)ベンズアルデヒド 溶液, Kovac法によるインドール試薬
Empirical Formula (Hill Notation):
C9H11NO
CAS Number:
Molecular Weight:
149.19
Beilstein:
4132845
MDL番号:
PubChem Substance ID:
NACRES:
NA.85

シェルフライフ

limited shelf life, expiry date on the label

品質水準

100

組成

n-butanol
4-dimethylaminobenzaldehyde
hydrochloric acid

technique(s)

microbe id | specific enzyme detection: suitable

屈折率

n20/D 1.417

application(s)

agriculture
clinical testing
environmental
food and beverages

microbiology

保管温度

2-8°C

適合性

Escherichia coli
coliforms

SMILES string

[H]C(=O)c1ccc(cc1)N(C)C

InChI

1S/C9H11NO/c1-10(2)9-5-3-8(7-11)4-6-9/h3-7H,1-2H3

InChI key

BGNGWHSBYQYVRX-UHFFFAOYSA-N

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詳細

Kovac′s reagent for indoles is a solution of 4-(dimethylamino)benzaldehyde and hydrochloric acid in n-butanol.

アプリケーション

Kovac′s reagent for indoles has been used for enumerating E. coli O157:H7 in ground beef samples in different scenarios of stored, thawed, and as cooked patty samples. It has also been used for E coli enumeration in dewatered blackwater.

生物化学的/生理学的作用

Kovac′s reagent detects the indole and aids in identifying the indole-positive and indole-negative microorganisms. E. coli can split tryptophan into indole and α-aminopropionic acid in the presence of the enzyme tryptophanase. p-Aminobenzaldehyde present in the reagent reacts with indole to form a cherry-red complex, that is soluble in alcohol, ether, and chloroform. It is recommended to use glucose free media and peptone with a high tryptophan content for the test.

ピクトグラム

FlameCorrosionExclamation mark

シグナルワード

Danger

危険有害性の分類

Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Irrit. 2

保管分類コード

3 - Flammable liquids

WGK

WGK 1

引火点(°F)

96.8 °F

引火点(℃)

36 °C

個人用保護具 (PPE)

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Certificate of Analysis

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Certificate of Origin

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