If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
P1379
TWEEN® 20
viscous liquid
別名:
ポリエチレングリコールソルビタンモノラウラート, ポリオキシエチレンソルビタンモノラウラート
サイズを選択してください
| 容量 | カタログ番号 | 在庫状況 | 単価 |
|---|
この商品について
description
non-ionic
Quality Level
assay
>40% (GC)
form
viscous liquid
mol wt
~1228
composition
lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids)
concentration
≥40.0% (GC)
technique(s)
DNA extraction: suitable, RNA extraction: suitable, cell culture | mammalian: suitable, protein purification: suitable
impurities
≤3.0% water
refractive index
n20/D 1.468 (lit.)
CMC
0.06 mM (20-25°C)
acid number
≤2.2 mg/g
hydroxyl value
96‑108 mg/g
transition temp
cloud point 76 °C
density
1.095 g/mL at 25 °C (lit.)
HLB
16.7
suitability
complies for IR spectroscopy
application(s)
advanced drug delivery
SMILES string
CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO
InChI
1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3
InChI key
HMFKFHLTUCJZJO-UHFFFAOYSA-N
General description
親水性成分と疎水性成分をあわせ持つ界面活性剤は、2つの主要なカテゴリー、すなわち水中でイオン化するイオン性（陰イオン性、陽イオン性、または両性）およびイオン化しない非イオン性に分類されます。当該製品は非イオン性界面活性剤であり、乳化、分散、可溶化などのさまざまな用途があり、洗浄剤としても使用されます。さらに、膜タンパク質の可溶化剤としても使用できます。
Application
TWEEN 20 は、一般的な濃度 0.05％で、膜ベースのイムノアッセイにおけるブロッキング剤として使用されています。TWEEN 20 は、0.005 ～ 0.5％の濃度で、哺乳類細胞の可溶化に使用できます。
- ウェスタンブロッティングにおける洗浄およびブロッキングバッファー
- 免疫組織化学におけるブロッキングバッファー
- ELISA（酵素結合免疫吸着アッセイ）における洗浄バッファー[1]
- PCR（ポリメラーゼ連鎖反応）における反応混合物
Features and Benefits
- ライフサイエンスおよび生化学に関する研究のための汎用性の高い界面活性剤
- 優れた可溶化剤、分散剤、かつ乳化剤
- DNA/RNA抽出、哺乳動物の細胞培養、およびタンパク質精製に適している
Packaging
各キットには100 mLのサンプルが3個入っており、各サンプルは別の生産ロットから取っています。
Preparation Note
Legal Information
1 of 1
当該品目 | |||
|---|---|---|---|
| suitability complies for IR spectroscopy | suitability - | suitability - | suitability - |
| form viscous liquid | form viscous liquid | form viscous liquid | form viscous liquid |
| technique(s) DNA extraction: suitable, cell culture | mammalian: suitable, RNA extraction: suitable, protein purification: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable | technique(s) DNA extraction: suitable, RNA extraction: suitable, cell culture | mammalian: suitable, protein purification: suitable |
| assay >40% (GC) | assay >40% (GC) | assay - | assay - |
| CMC 0.06 mM (20-25°C) | CMC 60 mg/L | CMC 0.06 mM (20-25°C) | CMC - |
| density 1.095 g/mL at 25 °C (lit.) | density 1.095 g/mL at 25 °C ((lit.)), 1.095 g/mL at 25 °C (lit.) | density 1.095 g/mL at 25 °C (lit.), 1.1 g/cm3 at 25 °C (lit.) | density 1.11 g/mL at 20 °C, 1.095 g/mL at 25 °C (lit.) |
Still not finding the right product?
Explore all of our products under TWEEN® 20
保管分類
10 - Combustible liquids
flash_point_f
527.0 °F - Pensky-Martens closed cup
flash_point_c
275 °C - Pensky-Martens closed cup
ppe
Eyeshields, Gloves
プロトコル
This page shows how to solubilize membrane proteins with products from Cytiva.
資料
Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| P1379-100ML | 04061837602108 |
| P1379-1KT | 04061842429400 |
| P1379-4L | 04061834354611 |
| P1379-6X1L | 04061837602160 |
| P1379-25ML | 04061837602139 |
| P1379-250ML | 04061838672940 |
| P1379-6X500ML | 04061837602177 |
| P1379-1L | 04061837602115 |
| P1379-500ML | 04061837602153 |
-
How can I determine the shelf life / expiration / retest date of this product?
1 回答-
役に立ちましたか？
-
-
What is the storage temperature of Tween 20?
1 回答-
This product may be stored at room temperature.
役に立ちましたか？
-
-
How is shipping temperature determined? And how is it related to the product storage temperature?
1 回答-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
役に立ちましたか？
-
-
What is the minimum dilution ratio of Tween20 in Exosome incubating buffer based on CMC level?
1 回答-
This product is not tested for any specific application. The critical micelle concentration of TWEEN 20 has been reported as 60 milligrams per liter. Please see the link below to review a publication that may be helpful:
https://open.clemson.edu/cgi/viewcontent.cgi?article=5274&context=all_theses役に立ちましたか？
-
-
Please provide the information on product container and material for P1379-500ML TWEEN® 20
1 回答-
This item is packaged in a 500 ML HDPE Bottle with a red PP cap w/ PTFE liner.
役に立ちましたか？
-
-
You state 0.06 mM as the CMC for this product. What is the stock concentration (in Molar) for this product? Alternatively what is the % to reach that 0.06 mM / CMC concentration? Thank you
1 回答-
The critical micelle concentration (CMC) reported on the product page is a value reported from the literature. This product is a neat or pure liquid. The base molecular weight of this material is 1,225 daltons and the density is 1.095 g/mL. However, this is based on the assumption of 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid. Please see the link below to review the general Tween 20 datasheet, note that the CMC value listed is 60 mg/L.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/128/958/p9416pis.pdf役に立ちましたか？
-
-
What is the grade/agency of Tween 20?
1 回答-
This product is a research grade TWEEN 20.
役に立ちましたか？
-
-
What is the expiry date once opened?
1 回答-
Long-term stability studies have not been performed, however, providing that the product is stored and handled properly, the expected shelf life is not expected to change. Factors such as improper closure and cross-contamination would certainly impact the overall stability and shelf life.
役に立ちましたか？
-
-
The data sheet for my lot says this is 50% pure. Does that mean it is 50% tween 20?
1 回答-
The 50% is referring to the concentration of lauric acid which occurs in the highest concentration. Fatty acid constituents of this product are determined by transesterification to yield fatty acid methyl esters, which are identified by gas chromatography. Fatty acids that are typically present in the offered Tween products are not a quantitative measurement. These include myristic, palmitic, and stearic acid, for the most part. While the Tween is considered a 'pure' product, the molecular weight is calculated on the assumption of 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid.
役に立ちましたか？
-
-
What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
役に立ちましたか？
-