In catalog number FLAAS, the specification for our vial Content is 0.90 - 1.10 mg ATP/vial by Enzymatic Assay. Please refer to the specific lot certificate of analysis for an individual quantity.
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製品名
アデノシン 5′-三リン酸 二ナトリウム塩 水和物, Grade I, ≥99%, from microbial
SMILES string
O.[Na+].[Na+].Nc1ncnc2n(cnc12)[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)[C@@H](O)[C@H]3O
InChI
1S/C10H16N5O13P3.2Na.H2O/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(26-10)1-25-30(21,22)28-31(23,24)27-29(18,19)20;;;/h2-4,6-7,10,16-17H,1H2,(H,21,22)(H,23,24)(H2,11,12,13)(H2,18,19,20);;;1H2/q;2*+1;/p-2/t4-,6-,7-,10-;;;/m1.../s1
InChI key
NTBQNWBHIXNPRU-MSQVLRTGSA-L
biological source
microbial
type
Grade I
assay
≥99%
form
powder
solubility
H2O: 50 mg/mL
storage temp.
−20°C
Quality Level
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関連するカテゴリー
1 of 4
当該品目 | A6419 | FLAAS | A3377 |
|---|---|---|---|
| biological source microbial | biological source microbial | biological source bacterial (Corynebacterium sp) | biological source microbial |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| assay ≥99% | assay ≥99% (HPLC) | assay ≥90% | assay ≥97% (HPLC) |
| form powder | form powder | form lyophilized powder | form crystalline |
| solubility H2O: 50 mg/mL | solubility H2O: 50 mg/mL | solubility - | solubility H2O: 50 mg/mL |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Application
Biochem/physiol Actions
保管分類
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type N95 (US)
プロトコル
AMP(アデノシン一りん酸)とATP(アデノシン三りん酸)の分離
Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)
Measure hexokinase activity using a continuous spectrophotometric rate-determination assay at 340 nm, catalyzing D-hexose sugar phosphorylation using ATP.
Measure luciferase activity using a luminometer assay detecting light emission, with applications in ATP detection and genetic function reporting.
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I would like to know how many g of ATP disodium salt hydrate contains in FLAAS -1vol vial? Thanks
1 回答-
役に立ちましたか?
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What volume of water should 1mg ATP be reconstituted in & what would be its corresponding concentration?
1 回答-
This product is soluble in water up to 50 mg/mL. The appropriate stock concentration for assays can be determined by the end user. The molecular weight is 551.14 (anhydrous basis), and the water content can be found on the lot-specific certificate of analysis. Using the molecular weight, the water content, and the chosen concentration for the ATP solution, the molarity can be calculated.
役に立ちましたか?
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Can I dissolve this ATP in water and store it?
1 回答-
This item can be solubilized in water at 50 mg/mL yielding a clear, colorless solution. Aqueous solutions of ATP are stable for months at -15C and or one week when stored at -0C as mentioned in the Product Information Sheet.
For more information, please navigate to the ‘DOCUMENTATION’ section to access the Product Information Sheet under 'More Documents':
https://www.sigmaaldrich.com/product/sigma/a2383#product-documentation役に立ちましたか?
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