This product is a 1.8 mM solution of ABTS
A3219
2,2′-アジノ-ビス(3-エチルベンゾチアゾリン-6-スルホン酸)
peroxidase substrate, chromogenic, liquid
別名:
ABTS
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| 容量 | カタログ番号 | 在庫状況 | 単価 |
|---|---|---|---|
| 100 mL | 在庫from市川市大町368 | ￥23,300 |
この商品について
￥23,300
在庫詳細
製品名
2,2′-アジノ-ビス(3-エチルベンゾチアゾリン-6-スルホン酸), Liquid Substrate System
Quality Level
form
liquid
storage temp.
2-8°C
SMILES string
CCN1C(\Sc2cc(ccc12)S(O)(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S(O)(=O)=O
InChI
1S/C18H18N4O6S4/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28)/b19-17-,20-18-
InChI key
ZTOJFFHGPLIVKC-CLFAGFIQSA-N
General description
生成物は、酸性緩衝液中の2,2′-アジノ-ビス(3-エチルベンズ-チアゾリン-6-スルホン酸) からなる、すぐに使用できるパーオキシダーゼ用1成分基質として提供されます。基質はパーオキシダーゼと反応する前は無色であり、反応後は青緑色の反応生成物を生成します。終点アッセイ中の反応を止めるために1%のドデシル硫酸ナトリウム（SDS）が使用されることがあります。この基質は可溶性反応生成物を生成するため、免疫ブロット・アプリケーションや組織化学的アプリケーションにはお勧めしません。
Application
Biochem/physiol Actions
2,2′-アジノ-ビス(3-エチルベンゾチアゾリン-6-スルホン酸)（ABTS）は陽イオンであり、過酸化水素存在下で酸化されます。酸化したABTSは青緑色の生成物であり、比色分析によって測定できます。ABTS、過酸化水素、およびミオグロビンの組み合わせは、試料から総抗酸化能（TAC）を測定するのに採用される標準系です。ABTS法はさまざまなpHに適応し、最も広く使用されています。ABTSはフェノール酸化酵素用の基質です。
Physical form
そのままお使いいただけます。
1 of 1
当該品目 | |||
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| form liquid | form powder | form tablet | form solution |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. room temp | storage temp. 2-8°C |
保管分類
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
資料
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
関連コンテンツ
Datasheet
A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation
Erel O
Clinical Biochemistry, 37(4), 277-285 (2004)
The opioid antagonist, beta-funaltrexamine, inhibits chemokine expression in human astroglial cells.
Randall L Davis et al.
Journal of neuroimmunology, 186(1-2), 141-149 (2007-05-04)
Emerging evidence indicates that neuroinflammatory responses in astroglia, including chemokine expression, are altered by opioids. Astroglial chemokines, such as CXCL10, are instrumental in response to many neuropathological insults. Opioid mediated disruption of astroglial CXCL10 expression may be detrimental in opioid
Comparison of DPPH and ABTS assays for determining antioxidant potential of water and methanol extracts of Spirulina platensis
Shalaby EA and Shanab SMM
Indian Journal of Geo-Marine Sciences, 42(5), 556-564 (2013)
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| A3219-100ML | 04061838251572 |
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what is the molarity of this solution?
1 回答-
役に立ちましたか？
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Can this product be used for antioxidant assay?
1 回答-
This product has not been specifically tested for use in antioxidant assays. However, it has been used successfully as such in several publications. Please see the link below to review one example:
https://www.sciencedirect.com/science/article/pii/S0009912003002315?via%3Dihub役に立ちましたか？
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What is the approximate concentration of H2O2 in the ready-to-use A3219 solution?
1 回答-
This product is no longer tested for hydrogen peroxide concentration. The previous specification for H2O2 was </= 0.03%. Current lots are expected to be within this range.
役に立ちましたか？
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What is the molecular weight and density?
1 回答-
This product is a 1.8 mM solution of ABTS, the density is not determined. The solution is prepared from a diammonium salt form with a molecular weight of 548.60.
Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/338/745/a3219dat.pdf役に立ちましたか？
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ABTS ready to use를 사용하여 항산화 실험을 하는경우 기존의 방법에 적용하면 어떤 단계가 생략이 되는건지 자세한 실험 과정을 알고 싶습니다
1 回答-
The only protocol currently available on the website is this data sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/338/745/a3219dat.pdf.
It may be helpful to also view the "Peer-Reviewed Papers" related to this product: https://www.sigmaaldrich.com/search/a3219?focus=papers&page=1&perpage=30&sort=relevance&term=a3219&type=citation_search.
役に立ちましたか？
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When using Product A3219, 2,2´-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), at what wavelength should the product of the reaction be measured?
1 回答-
The product should be measured at 405 nm. For end point assays, the reaction may be stopped with 1% (w/v) SDS and also read at 405 nm.
役に立ちましたか？
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What is the concentration of Product A3219, 2,2´-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)?
1 回答-
The concentration of the ABTS is approximately 1.8 mM.
役に立ちましたか？
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Should Product A3219, 2,2´-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), be diluted prior to use?
1 回答-
No, this product is offered as a ready-to-use (1×) solution.
役に立ちましたか？
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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