As per the Product Information Sheet, the Accutase solution does not contain mammalian or bacterial-derived products. This solution is supplied as a sterile, ready-to use, frozen liquid. This product is serum free.
A6964
アクターゼ 溶液
sterile-filtered, suitable for cell culture
別名:
Cell detachment solution
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当該品目 | SCR005 | SCR104 | A7089 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable, single cell analysis: suitable |
| form liquid | form liquid | form liquid | form solution |
| concentration 400-600 units/mL | concentration - | concentration - | concentration 1 × |
| pH 6.8-7.8 | pH - | pH - | pH 6.8-7.8 |
| sterility sterile-filtered | sterility - | sterility - | sterility sterile-filtered |
| storage temp. −20°C | storage temp. - | storage temp. - | storage temp. −20°C |
General description
アキュターゼ®溶液［Ca++またはMg++を含まないダルベッコリン酸緩衝生理食塩水（DPBS）］は、細胞剥離のためのタンパク質分解酵素およびコラーゲン分解酵素を含有しています。標準的な組織培養プラスチック製品や接着コーティングされたプラスチック製品からの細胞剥離に有用です。アキュターゼは、細胞を少ない損傷で培養容器から剥離するために役立つため、細胞剥離に関してはトリプシンよりも推奨されます。そのため、アキュターゼは、感受性細胞の処理や表面マーカーの分析のためによく用いられます。
Application
アキュターゼ®溶液は、脳腫瘍細胞[1]、人工多能性幹細胞（IPSC）[2]および膠芽腫細胞[3]を単一細胞に分散させるなど、凝集したクラスターの中で増殖する非付着細胞の剥離や消化に使用されています。
シーケンシングのための単一細胞懸濁液の調製用に適しています。
シーケンシングのための単一細胞懸濁液の調製用に適しています。
一次線維芽細胞、内皮細胞、ニュ-ロン、腫瘍細胞株、昆虫細胞の剥離に有効であることが証明されています。 細胞表面マ-カ-の分析、ウイルス成長アッセイ、フロ-サイトメトリ-およびバイオリアクタ-のスケ-ルアップに使用する細胞の剥離に、非常に有用です。 哺乳類または微生物に由来する産物を含んでいません。
Features and Benefits
タンパク質分解酵素とコラ-ゲン分解酵素からなる独自組成の混合液です。標準的なプラスチック容器や接着被膜されたプラスチック容器からの細胞の剥離にそのままお使いいただけます。
Preparation Note
0.5 mM EDTA•4Na、3 mg/L フェノールレッド含有ダルベッコPBS(0.2 g/L KCl、0.2 g/L KH2PO4、8 g/L NaCl、1.15 g/L Na2HPO4)で調製。製品の使用法情報ウェブサイト上の製品データシートをご参照ください。
本製品は、フェノールレッドを含んでいます。 本製品は、ドライアイスと共に出荷されるため、パッケージ内に相当量の CO2 が蓄積することがあります。この CO2 は、溶液内に侵入し、わずかに pH を下げ、黄色-オレンジ色（pH 6.8）を生成することがあります。この溶液はそのままでも使用できますが、pH を調整するために水酸化ナトリウムを添加しても構いません。完全に凍結した Accutase のボトルは黄色ですが、解凍するとオレンジ色に変化します。一度開封した製品は、再凍結することも、直接冷蔵庫に入れて後で使用することもできます。 暖かいウォーターバスでこの製品を解凍ないでください。Accutase は 37 °C 以上の温度に敏感であり、37 °C で 45 分間置くと不活性化します。冷蔵庫で一晩かけて解凍するか、1～1.5 時間ほど水道水の入った容器で解凍することをお勧めします。 解凍後は、成分が均一に溶解するように、Accutase のボトルを振ってください。この製品は、使用前に温める必要はありません。冷蔵庫から取り出したら速やかに使用し、使用後は冷蔵庫に戻してください。
Legal Information
Accutase is a registered trademark of Innovative Cell Technologies, Inc.
Related product
保管分類
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
A6964-VAR: + A6964-100ML: + A6964-500ML-LBL: + A6964-100ML-LBL: + A6964-500ML: + A6964-BULK: + A6964-500ML-PW: + A6964-100ML-PW:
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Is Accutase Solution A6964 animal-origin free? Is serum free?
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Can accutase be thawed, aliquoted, and refrozen at -20 to preserve it longer?
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Yes, this product is stable after undergoing several freeze/thaw cycles. The thawed product may be aliquoted and stored at –20 °C or stored for up to 2 months at 4°C.
The graphic below demonstrates the freeze/thaw stability of Accutase.役に立ちましたか？
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On the Innovative cell technologies website it says that accumax is the same as accutase but with 3x the amount of enzymes. Why did the technician here answer that it contains DNAse?
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Thank you for calling this to our attention. The manufacturer, Innovative Cell Technologies, has been contacted regarding this matter. The manufacturer has confirmed that both Accutase and Accumax products DO contain a small amounts of DNAse. The concentration is not measured, but it is present in the formulation. In addition, both products also contain 0.5 mM EDTA.
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Will Product A6964, Accutase® solution, work on tissue?
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This product is not tested for tissue, but it should work on soft tissue.
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Does Product A6964, Accutase® solution, contain animal components?
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Accutase is purified from a marine invertebrate. There are no other animal or bacterial derived proteins present.
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What is the appropriate cell dissociating product to be used for ES cells that will be injected into blastocysts?
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Accutase®, product A6964, or Accumax™ (Accutase® with DNase), product A7089, will work well for dissociation of ES cells. The Accutase® product has protease and dispase activity to dissociate the cells. The Accumax™ product also has DNase to limit the aggregation that is caused by DNA released from ruptured cells during the dissociation/scraping.Please see the reference and link below for more information on the use of Accutase® with ES cells:Mol Reprod Dev. 2008 May;75(5):818-27
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How can I inactivate Accutase® with fetal bovine serum?
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Due to its gentle nature Accutase® cell detachment solution usually does not need to be neutralized following cell passaging. Accutase® is also heat inactivated after 45 minutes at 37°C. If this method of neutralization does not work for your cells, or if you want to neutralize Accutase® in a shorter period of time, the addition of 10% FBS containing media will accomplish this task. Experiments have shown that diluting a sample of Accutase® with 10% with fetal bovine serum achieved an environment similar to that found in cell culture. Measurements of the enzyme activity in this diluted sample showed decreased activity over time, falling below that of the required level for cell detachment (>175 units/mL). After about 6 minutes of serum interaction, the enzyme activity of Accutase® was well below this level, demonstrating effective inhibition by FBS.
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For what purposes can Product A6964, Accutase® solution, be used?
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It can be used for detaching cells, analysis of cell surface markers and virus growth assays.
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What is the concentration of Product A6964, Accutase® solution?
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This product is typically at a concentration of 400-600 units/mL.
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What is the difference between Accutase and Accumax?
1 回答-
Accutase is a mixture of non-mammalian proteases. Accumax is accutase that has been augmented with DNase to reduce clumping associated with DNA release from damaged cells.
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