A6982

Sigma-Aldrich

HydroMatrix ペプチド細胞培養スカフォールド

mixture, powder

品質水準

100

由来生物

synthetic

形態

powder

保管温度

−20°C

類似した製品をお探しですか? Visit 製品比較ガイド

アプリケーション

HydroMatrix has been shown to support improved growth and migration in the proliferation of many cell types, including neural stem cells, neurons, glia, astrocytes, fibroblasts, and keratinocytes.

構成

HydroMatrix is a peptide nanofiber 3-dimensional scaffold that promotes cell growth and migration. It utilizes specific peptides that self-assemble from fluid precursors into highly crosslinked peptide hydrogels when presented with increased temperatures or ionic strengths. By inducing a rapid transformation from solution to gel, a peptide nanofiber scaffold is generated. Adjusting the concentration of the HydroMatrix solution, researchers can control the flexibility of the 3-D architecture to meet their application′s specific needs.

注意

The lyophilized product at -20°C and is stable for many years at this temperature. The hydrogel should be stored at -70°C to maximize its active span. The 1% (w/v) stock solution should be stored -20°C and is stable for 3-6 months.

調製ノート

This product is supplied as a lyophilized powder. A 1% (w/v) stock solution at 10 mg/mL can be prepared with addition of sterile water, resulting in a rigid gel. A 0.5% (w/v) solution will lead to a softer gel that is more suitable for cell migration and angiogenesis. Lower concentrations in the range of 0.15%-0.25% are more suitable for many cell types including neurons. In aqueous solution, HydroMatrix has a pH of 2.5 and should not be added directly to cells without prior addition of a buffer or medium. Once the hydrogel has formed, do not attempt to mix the gel any further, as this risks destroying the hydrogel integrity. Manipulation of the cells should be carefully performed to avoid disruption of the scaffold.

法的情報

HydroMatrix is a trademark of Sigma-Aldrich Co. LLC

storage_class_code

13 - Non Combustible Solids

ドイツ水質汚染分類

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable

個人用保護具 (PPE)

Eyeshields, Gloves, type N95 (US)

試験成績書(COA)

原産地証明書(COO)

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What volume of H2O should be used for reconstitution of Product A6982, HydroMatrix Peptide Cell Culture Scaffold?

    The package size of the A6982 indicates the volume of H2O to use for reconstitution.  For example, a 1 ml package will require 1 ml of  H2O for reconstitution.

  6. Will Product A6982, HydroMatrix Peptide Cell Culture Scaffold, work will for adherent cells and suspension cells?   

    Yes, the product works with both cell types.

  7. What is the concentration of Product A6982, HydroMatrix Peptide Cell Culture Scaffold, when reconstituted as indicated?

    A 1% (w/v) (10 mg/ml) stock solution is accomplished by the addition of sterile dH20 as indicated.

  8. What concentration of HydroMatrix Peptide Scaffold, Product A6982, should I use for my cells?

    A 1% (w/v) solution results in a stronger gel. A working concentration of 0.5% (w/v) results in a soft gel with weak mechanical strength that is suitable for cell migration and angiogenesis.  Working solution concentrations as low as 0.15-0.25% are suitable for many cell types, including neurons. It is recommended for most applications to start with a 0.25% (w/v) working solution (2.5 mg/ml).

  9. How do I embed or encapsulate cells in HydroMatrix Peptide Scaffold, Product A6982, before polymerization?

    In a solution of dH20, HydroMatrix has a pH of 2.0-2.5; therefore it must not be added directly to cells.  The addition of buffer or media is performed prior to the addition of cells.  If performing cell encapsulation studies, addition of iso-osmotic sucrose will help protect the cells until a physiological pH is obtained.

  10. How does HydroMatrix Peptide Cell Culture Scaffold compare to PuraMatrix from Becton-Dickenson?

    Both products are peptide hydrogels.  There is no similarity between the peptides used to create the matrices.  They are both just classified as self-assembling peptide hydrogels. The sequence for the peptide is considered proprietary.

Amish Asthana et al.
Drug discovery today, 18(11-12), 533-540 (2012-12-29)
Force and substrate physical property (pliability) is one of three well established microenvironmental factors (MEFs) that may contribute to the formation of physiologically more relevant constructs (or not) for cell-based high-throughput screening (HTS) in preclinical drug discovery. In 3D cultures...
Amish Asthana et al.
Drug discovery today, 17(15-16), 810-817 (2012-04-10)
The three microenvironmental factors that characterize 3D cultures include: first, chemical and/or biochemical composition, second, spatial and temporal dimensions, and third, force and/or substrate physical properties. Although these factors have been studied individually, their interdependence and synergistic interactions have not...
Daniela Stoppoloni et al.
Rheumatology international, 33(9), 2399-2403 (2012-03-28)
Osteoarthritis (OA) is one of the most common degenerative joint disease for which there is no cure. It is treated mainly with non-steroidal anti-inflammatory drugs to control the symptoms and some supplements, such as glucosamine and chondroitin sulphate in order...
Josine E G Vaes et al.
Glia, 69(3), 655-680 (2020-10-13)
Encephalopathy of prematurity (EoP) is a common cause of long-term neurodevelopmental morbidity in extreme preterm infants. Diffuse white matter injury (dWMI) is currently the most commonly observed form of EoP. Impaired maturation of oligodendrocytes (OLs) is the main underlying pathophysiological...
資料
3D細胞培養の概要2D細胞培養と3D細胞培養の比較、3D細胞培養の利点、3D細胞培養技術について紹介します。
さらに詳しく
3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models
さらに詳しく
Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)
さらに詳しく

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

テクニカルサービス連絡先