Solutions should be stored in single use aliquots at -20ºC. Frozen aliquots should be stable for at least 6 months.
C4951
コレステロール-水溶性
powder, BioReagent, suitable for cell culture
Cholesterol-Water Soluble
別名:
コレステロール–メチル-β-シクロデキストリン
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| 容量 | カタログ番号 | 在庫状況 | 単価 |
|---|---|---|---|
| 30 mg | 在庫あり出荷可能from市川市大町368 | ￥13,500 |
この商品について
NACRES:
NA.75
UNSPSC Code:
12352211
biological source
ovine wool
Quality Level
product line
BioReagent
form
powder
technique(s)
cell culture | mammalian: suitable
solubility
H2O: 300 mg/mL
shipped in
ambient
storage temp.
−20°C
Application
水溶性コレステロールは以下のように用いられています：
- ラット平滑筋細胞（SMCs）のフリーコレステロール過重負荷に
- 細胞毒性測定に
- Helicobacter pyloriとCD4+T細胞のインキュベーションに[1]
- SH-SY5Yヒト神経芽腫細胞中の膜コレステロールレベルの研究に
- 細胞の生存能力に与えるコレステロールの影響、および初期培養ラット海馬ニューロン中3-（4,5-ジメチルチアゾール-2-イル）-2,5-ジフェニルテトラゾリウムブロミド（MTT）の還元の研究に
- 細菌培養の増殖サプリメントとして
- Sタンパク質とアンジオテンシン変換酵素2（ACE2）相互作用の調査
- モルモットの内有毛細胞内のカリウム流に与えるコレステロールの影響に関する研究
- プロテナーゼKの可逆阻害に
Packaging
容量はコレステロ-ルの量に基づいています。
Preparation Note
300 mg/mLの水溶液を調製します。 希釈後1時間溶液を静置し、生成物を完全に可溶化させます。
Other Notes
1 gあたり約40 mgのコレステロ-ルが含まれ、残りはメチル-β-シクロデキストリンが配合されています。
1 of 1
当該品目 | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable |
| biological source ovine wool | biological source ovine wool | biological source - | biological source non-animal source |
| shipped in ambient | shipped in ambient | shipped in dry ice | shipped in - |
| form powder | form powder | form solid | form aqueous solution |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| solubility H2O: 300 mg/mL | solubility H2O: 0.002 mg/mL, Tween: soluble, ethanol: soluble, serum: soluble | solubility H2O: 60 mg/mL | solubility - |
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Importance of cholesterol-rich membrane microdomains in the interaction of the S protein of SARS-coronavirus with the cellular receptor angiotensin-converting enzyme 2
Glende J, et al.
Virology, 381(2), 215-221 (2008)
Cholesterol and its derivatives reversibly inhibit proteinase K
Singh N and Bhattacharyya D
Journal of Cellular Physiology, 232(3), 596-609 (2017)
A novel reciprocal and biphasic relationship between membrane cholesterol and beta-secretase activity in SH-SY5Y cells and in human platelets
Liu WW, et al.
Journal of Neurochemistry, 108(2), 341-349 (2009)
グローバルトレードアイテム番号
| カタログ番号 | GTIN |
|---|---|
| C4951-30MG | 04061833494110 |
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How stable is this product when dissolved in water to 300 mg/mL? Can it be frozen for long-term storage?
1 回答-
役に立ちましたか？
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What is the molecular weigth of this specific product? Can you share and add g/mol vale please?
1 回答-
The molecular weight of cholesterol used for this product is 386.65 g/mol. For the calculation of molarity based on cholesterol content, approximately 0.4 mg of the 10 mg vial content is cholesterol. The lot-specific Certificate of Analysis should be checked to find the lot-specific cholesterol content for the received product.
役に立ちましたか？
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Hi, When you say it has biphasic solubility, I understand what you mean, but in that, when you refer to the mg, do you mean the mg of cholesterol WITHIN the compound methyl-B-cyclodextrin, or the mg of the entire compound?
1 回答-
The reference to mg pertains to the amount of solid powder used, which is 300 mg of the product per mL of water. It is important to allow the solution to sit undisturbed for one hour after dilution to achieve complete solubilization of the product.
役に立ちましたか？
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Is there a minimum or maximum solubility limit for this Cholesterol-Cyclodextrin product?
1 回答-
Due to the nature of cyclodextrin, the cholesterol-cyclodextrin complex exhibits biphasic solubility. To ensure optimal solubility at low concentrations, it is suggested that stock solutions be prepared at a minimum of 50 mg/mL Note that the optimum suggested stock solution concentration is 100 – 300 mg/mL. This material has not been tested for solubility at concentrations greater than 300 mg/mL.
役に立ちましたか？
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How long can the cholesterol powder maintain its potency when store at -20 and 4 degree, respectively?
1 回答-
This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date are subject to a one-year warranty from the date of shipment. For more information, you may access the Product Dating Information document under ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
More details for product solubility and storage can be found in this link below:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/218/859/e8014inf.pdf役に立ちましたか？
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For how long can the powder be stored at -20C?
1 回答-
This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one-year warranty from the date of shipment. For more information, you may access the Product Dating Information document under ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
役に立ちましたか？
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Is it appropriate to test MbCD as a vehicle when using this product for cell treatment?
1 回答-
Methyl-beta-cyclodextrin, catalog number C4555, would be an appropriate choice to use as a vehicle treatment. More information can be found at https://www.sigmaaldrich.com/product/sigma/c4555.
役に立ちましたか？
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Hay algo que no entiendo respecto a este producto. Se asegura que el contenido es de 40 mg de colesterol por g de producto, sin embargo el envase es de 30 mg, eso quiere decir que la concentración de colesterol en el envase es de 1,2 mg? no queda claro
1 回答-
The current specification for this product is that it contains greater than or equal to 40 mg of cholesterol per gram. Each vial contains 30 mg of cholesterol. Therefore, the total mass of the product will always be less than 750 mg.
役に立ちましたか？
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C4951 Cholesterol-Water Soluble 30 mg. Technical Support online shows: The 30 mg package size may contain nearly 1 gram of powder. so, what's the other substance besides the 30 mg of the active ingredients?
1 回答-
The bulk of this material is Methyl-?-cyclodextrin. This component enhances the solubility of Cholesterol in water. There are approximately 40 mg of Cholesterol per gram of product, with the remainder being the cyclodextrin agent. Cyclodextrins are not cell permeable and inert in culture. Please see the link below to review additional information regarding these water-soluble complexes:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/218/859/e8014inf.pdf役に立ちましたか？
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CAN CHOLESTEROL POWDER BE DISSOLVED IN Medium of cells (High glucose, 2%fbs, 1% p/s) ?
1 回答-
The product is tested for solubility in water at 300 mg of the powder per 1 mL. Reconstituting in medium or salt solutions may cause a precipitate and is not recommended. Stock solutions in water may then be filter sterilized and added directly to the culture medium. Note that this product is sold on the basis of the cholesterol content only. The 30 mg package size may contain nearly 1 gram of powder. The specific amount of cholesterol per gram is reported on the lot-specific Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/c4951#product-documentationPlease see the links below to learn more about the water-soluble complexes:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/181/278/c4805inf.pdf
https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/mammalian-cell-culture/cyclodextrins役に立ちましたか？
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