Merck
All Photos(3)

C5670

Sigma-Aldrich

塩化カルシウム

anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%

Linear Formula:
CaCl2
CAS Number:
Molecular Weight:
110.98
EC 番号:
MDL番号:
PubChem Substance ID:
NACRES:
NA.71

グレード

anhydrous

品質水準

200

蒸気圧

0.01 mmHg ( 20 °C)

製品ライン

BioReagent

アッセイ

≥96.0%

形態

powder

technique(s)

cell culture | insect: suitable
cell culture | plant: suitable

mp

772 °C (lit.)

SMILES string

[Cl-].[Cl-].[Ca++]

InChI

1S/Ca.2ClH/h;2*1H/q+2;;/p-2

InChI key

UXVMQQNJUSDDNG-UHFFFAOYSA-L

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詳細

Calcium chloride is a commonly used reagent in biochemistry. Calcium plays important roles in many biological processes, including signal transduction, muscle contraction, and maintenance of cell membrane and cell wall stability.1,2 Extensive reviews of the experimental measurement of biological calcium have been published.3,4Calcium chloride is used in the preparation and transformation of competent E. coli and in the transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA.5 The CaCl2-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied.6 A protocol for the concentration of virus vectors that uses CaCl2 has been published.7CaCl2 has been used in the stabilization and two-dimensional crystallization of the NADH-ubiquinone oxidoreductase (complex I) from Escherichia coli.8 The crystallization of porcine pancreatic elastase in the presence of CaCl2 in the presence of sodium citrate reveals binding of calcium in the metal binding site of the protein.9 The effect of CaCl2 on the total fluorescence in the polymerication of the tubulin-like FtsZ division protein of Escherichia coli has been studied by a multiwell fluorescent assay.10

包装

100, 500 g in poly bottle

アプリケーション

This product is insect cell culture tested (0.5 mg/ml) and is appropriate for insect cell culture applications. Calcium chloride is used in the preparation and transformation of competent E. coli and in the
transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA. The CaCl2-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied. A protocol for the concentration of virus vectors that uses CaCl2 has been published.

調製ノート

This product is soluble in water (294 mg/ml, 2 M), yielding a clear, colorless solution.

ピクトグラム

Exclamation mark

シグナルワード

Warning

危険有害性情報

危険有害性の分類

Eye Irrit. 2

保管分類コード

13 - Non Combustible Solids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable

Certificate of Analysis

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Certificate of Origin

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