CMC0001 is classified as, "not a hazardous substance or mixture" in the GHS-US classification system. The E. coli is not a hazardous ingredient at levels requiring disclosure by the OSHA Hazard Communication Standard (29 CFR 1910.1200). We do not test for pathogens or pathogenic properties.
CMC0001
SIG10 Chemically Competent Cells
Escherichia coli, rod shaped
別名:
Chemically competent E. coli, Competent bacterial cells
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この商品について
UNSPSC Code:
41106202
NACRES:
NA.85
Biological source:
Escherichia coli
Growth mode:
adherent or suspension
Morphology:
rod shaped
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製品名
SIG10 Chemically Competent Cells, for protein expression and DNA plasmid production
biological source
Escherichia coli
grade
Molecular Biology
form
buffered aqueous solution
growth mode
adherent or suspension
morphology
rod shaped
technique(s)
microbiological culture: suitable
cell transformation
competent cell type: chemically competent
transformation efficiency: ≥1 × 109 cfu/μg
shipped in
dry ice
storage temp.
−70°C
Application
Suitable for bacterial transformations to recover high quality plasmid DNA
Biochem/physiol Actions
The SIG10 cells contains the inactive mcr and mrr alleles, allowing methylated genomic DNA isolated directly from mammalian or plant cells to be cloned without deletions or rearrangements.
This strain also carries the recA1 and endA1 mutations. The recA1 genotype provides minimized recombination and aids in plasmid stability while endA1 provides for high quality plasmid DNA preparation.
They are bacteriophage T1-resistant (tonA mutation) and also resistant to streptomycin by virtue of rpsL mutation.
This strain also carries the recA1 and endA1 mutations. The recA1 genotype provides minimized recombination and aids in plasmid stability while endA1 provides for high quality plasmid DNA preparation.
They are bacteriophage T1-resistant (tonA mutation) and also resistant to streptomycin by virtue of rpsL mutation.
Features and Benefits
- share the most useful genetic elements of standard cloning strains like DH5α™ DH10B, JM109, TOP10, etc. and directly replace them in cloning protocols.
- ensures recovery of stable and high quality plasmid DNA.
- high transformation efficiency
- provide the performance researchers need with ease of use.
- provide solutions for a wide range of applications at economical prices.
- Blue - white screening
General description
The SIG10 Chemically Competent Cells are a derivative of Escherichia coli that have been optimized for high transformation efficiency.
These cells are ideal for cloning and propagation of plasmid, cosmid, or fosmid clones and are highly efficient for routine cloning applications.
They share the most useful genetic elements of standard cloning strains like DH5α™ DH10B, JM109, TOP10, etc. and directly replace them in cloning protocols.
They are are provided in 40 μL, 80 μL and 160 μL aliquots, each being sufficient for one, two and four transformations respectively.
The cells have a transformation efficiency of >1 x 109 cfu/μg
The 96-well format are provided in aliquots of 20 μL per well and have a transformation efficiency of >1 x 108 cfu/μg.
Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA
These cells are ideal for cloning and propagation of plasmid, cosmid, or fosmid clones and are highly efficient for routine cloning applications.
They share the most useful genetic elements of standard cloning strains like DH5α™ DH10B, JM109, TOP10, etc. and directly replace them in cloning protocols.
They are are provided in 40 μL, 80 μL and 160 μL aliquots, each being sufficient for one, two and four transformations respectively.
The cells have a transformation efficiency of >1 x 109 cfu/μg
The 96-well format are provided in aliquots of 20 μL per well and have a transformation efficiency of >1 x 108 cfu/μg.
Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA
Other Notes
- SIG10 chemically competent cells
- pUC 19 transformation control DNA at 10 pg/μL
- recovery medium for cloning
Legal Information
DH5α is a trademark of Invitrogen Corp.
related product
製品番号
詳細
価格
保管分類
10 - Combustible liquids
資料
Colony PCR reagents and our colony PCR protocol using REDExtract-N-Amp™ PCR ReadyMix™ and JumpStart™ REDTaq® PCR ReadyMix™ reagents.
プロトコル
For best results, ligation reactions must be heat inactivated at 70º C for 15 minutes before transformation. Alternately, the reactions may be purified.
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Is it non pathogenic and biosefety level 1?
1 回答-
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Could you please clarify whether bacteria transformed with the pUC19 control provided with the SIG10 Competent Cells should grow colonies that are resistant to Ampicillin?
1 回答-
The colonies transformed with pUC19 as a control are resistant to ampicillin or methicillin, as indicated on the website.
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