すべての画像(1)

CS0740

Sigma-Aldrich

酸性ホスファターゼアッセイキット

 kit sufficient for 1,000 assays (multiwell plates),  kit sufficient for 100 assays (tubes)

eCl@ss:
42010102
NACRES:
NA.84

品質水準

200

使用法

1 kit sufficient for 1,000 assays (multiwell plates)
1 kit sufficient for 100 assays (tubes)

輸送温度

dry ice

保管温度

−20°C

Gene Information

human ... ACP1(52) , ACP2(53)

アプリケーション

組織、細胞抽出物、カラム画分や精製酵素の酸性ホスファタ-ゼ活性の検出用キットです。キットには迅速かつ簡便にホスファタ-ゼを検出するために必要な試薬がすべて揃っています。スタンダ-ド溶液とコントロ-ル酵素も含まれています。

生物化学的/生理学的作用

酸性ホスファタ-ゼは通常リソソ-ムに存在する酸性ヒドロラ-ゼの一種です。細胞成分を分画する際に、リソソ-ムを同定するマ-カ-となります。

キットの構成要素のみ

製品番号
詳細

  • p-Nitrophenyl phosphate tablets 5 mg 20 tablets

  • Citrate buffer solution, 0.09 M pH 4.8 100 mL

  • p-Nitrophenol Standard solution 10 mM 1 mL

  • Acid phosphatase control enzyme .2 mL

ピクトグラム

Skull and crossbonesHealth hazard

シグナルワード

Danger

危険有害性の分類

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Carc. 2 - STOT RE 2 Oral - STOT SE 1

ターゲットの組織

Eyes

保管分類コード

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable

試験成績書(COA)

原産地証明書(COO)

Qinghe Song et al.
PloS one, 10(3), e0120790-e0120790 (2015-03-27)
The Yes-associated protein (YAP), a transcriptional coactivator inactivated by the Hippo tumor suppressor pathway, functions as an oncoprotein in a variety of cancers. However, its contribution to breast cancer remains controversial. This study investigated the role of YAP in breast
Xiaogang Xu et al.
Frontiers in immunology, 9, 2321-2321 (2018-11-22)
Glycyrrhizin (GL), a triterpenoid glycoside, serves important functions in various biological activities, including antiviral and antitumor immune responses. However, the anti-inflammatory effects of GL on Salmonella enterica serovar Typhimurium (ST)-induced injury in mice and the mechanisms underlying the protection of
Lan Xiao et al.
OncoTargets and therapy, 9, 1105-1114 (2016-04-14)
To identify the role of YAP in cisplatin resistance in human ovarian cancer cells and in the regulation of autophagy in these cancer cells. The cisplatin-sensitive OV2008 parental cell line and its cisplatin-resistant variant C13K were cultured. RNA interference was
Jeffrey E McLean et al.
Journal of virology, 83(16), 8233-8246 (2009-06-06)
The ectopic overexpression of Bcl-2 restricts both influenza A virus-induced apoptosis and influenza A virus replication in MDCK cells, thus suggesting a role for Bcl-2 family members during infection. Here we report that influenza A virus cannot establish an apoptotic
Fan Zhang et al.
American journal of physiology. Cell physiology, 301(2), C421-C430 (2011-05-27)
It is well known that the mutation of TRP-ML1 (transient receptor potential-mucolipin-1) causes mucolipidosis IV, a lysosomal storage disease. Given that lysosomal nicotinic acid adenine dinucleotide phosphate (NAADP)-Ca(2+) release channel activity is associated with TRP-ML1, the present study was designed

資料

Organelle Isolation

The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify organelle fractions from gently homogenized samples.

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