D5921

DMEM - low glucose

Name: DMEM - low glucose
Brand: SIGMA
Price: 2832 JPY

With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

別名:

DME, DMEM

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500 ML

￥2,832

単価￥3,540割引20%

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この商品について

UNSPSC Code:

12352207

NACRES:

NA.75

MDL number:

MFCD00217342

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製品名

ダルベッコ改変イーグル培地 - 低グルコース, With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: no
sodium pyruvate: no
glucose: low
HEPES: no
L-glutamine: no
NaHCO₃: yes

shipped in

ambient

storage temp.

2-8°C

Quality Level

400

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当該品目	D2429	D5546	D5523
Sigma-Aldrich D5921 Dulbecco′s Modified Eagle′s Medium - low glucose	Sigma-Aldrich D2429 Dulbecco′s Modified Eagle′s Medium - low glucose	Sigma-Aldrich D5546 Dulbecco′s Modified Eagle′s Medium - low glucose	Sigma-Aldrich D5523 Dulbecco′s Modified Eagle′s Medium - low glucose
form liquid	form liquid	form liquid	form powder
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered	sterility -
components phenol red: no sodium pyruvate: no glucose: low HEPES: no L-glutamine: no NaHCO₃: yes	components NaHCO₃: no sodium pyruvate: yes phenol red: yes glucose: low L-glutamine: no HEPES: no	components NaHCO₃: yes phenol red: yes glucose: low HEPES: no sodium pyruvate: yes L-glutamine: no	components L-glutamine: yes NaHCO₃: no sodium pyruvate: yes phenol red: yes HEPES: no glucose: low
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient

Application

ダルベッコ改変イーグル培地低グルコースは、以下の培養で使用されています：′′

生きている大腸がんSW620細胞[1]
ヒト乳がん細胞株MCF-7(13)、KPL、およびMDA-MB-231[2]
哺乳類・イヌ由来間葉系幹細胞[2]

General description

ダルベッコ改変イーグル培地（DME）は基礎培地イーグル（BME）の改変型で、アミノ酸やビタミン、その他の補足成分の濃度が4倍になってます。′′最初のDME組成は、1,000 mg/Lのグルコース含有でマウス胚性細胞を培養することが報告されました。4,500 mg/Lのグルコースでの変更は、特定の細胞型の培養に最適です。DMEは、培地の安定性を改善することが示されています。

Other Notes

幹細胞または細胞クローンを扱う研究者は、フェノールレッドを含まない培地をよく好みます。

Preparation Note

0.584 g/LのL-グルタミンを添加してください。

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製品番号

詳細

価格

G7513

L-Glutamine solution, 200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture

P3532

Phenol Red, powder, BioReagent, suitable for cell culture

F2442

Fetal Bovine Serum, USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

pictograms

GHS07

signalword

Warning

hcodes

H317

pcodes

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

保管分類

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Treatment with 2-methoxyestradiol increases endothelial nitric oxide synthase activity via scavenger receptor class BI in human umbilical vein endothelial cells.

Tao Dong et al.

Molecular human reproduction, 26(6), 441-451 (2020-04-26)

Concentrations of 2-methoxyestradiol (2ME2), a principal metabolite of estradiol, are significantly lower in women with severe preeclampsia. Nitric oxide (NO) released by endothelial nitric oxide synthase (eNOS) plays an important role in regulating cardiovascular homeostasis. Importantly, high-density lipoprotein (HDL) stimulates

Biomechanics of subcellular structures by non-invasive Brillouin microscopy.

Giuseppe Antonacci et al.

Scientific reports, 6, 37217-37217 (2016-11-16)

Cellular biomechanics play a pivotal role in the pathophysiology of several diseases. Unfortunately, current methods to measure biomechanical properties are invasive and mostly limited to the surface of a cell. As a result, the mechanical behaviour of subcellular structures and

Photobiomodulation of human dermal fibroblasts in vitro: decisive role of cell culture conditions and treatment protocols on experimental outcome.

C Mignon et al.

Scientific reports, 7(1), 2797-2797 (2017-06-07)

Photobiomodulation-based (LLLT) therapies show tantalizing promise for treatment of skin diseases. Confidence in this approach is blighted however by lamentable inconsistency in published experimental designs, and so complicates interpretation. Here we interrogate the appropriateness of a range of previously-reported treatment

HMGA1a induces alternative splicing of estrogen receptor alpha in MCF-7 human breast cancer cells.

Kenji Ohe et al.

The Journal of steroid biochemistry and molecular biology, 182, 21-26 (2018-04-22)

The high-mobility group A protein 1a (HMGA1a) protein is known as an oncogene whose expression level in cancer tissue correlates with the malignant potential, and known as a component of senescence-related structures connecting it to tumor suppressor networks in fibroblasts.

Characterization of iCell cardiomyocytes using single-cell RNA-sequencing methods.

Christina Schmid et al.

Journal of pharmacological and toxicological methods, 106, 106915-106915 (2020-09-02)

Human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes are being evaluated for their use in pharmacological and toxicological testing, particularly for electrophysiological side effects. However, little is known about the composition of the commercially available iCell cardiomyocyte (Fuijifilm Cellular Dynamics) cultures

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