Merck
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E1270

Sigma-Aldrich

ECMゲル EHS肉腫由来

liquid, BioReagent, suitable for cell culture

別名:
EHSマトリックス
MDL番号:
NACRES:
NA.75

品質水準

300

製品ライン

BioReagent

形態

liquid

濃度

8-12 mg/mL

technique(s)

cell culture | mammalian: suitable

UniProtアクセッション番号

輸送温度

dry ice

保管温度

−20°C

アプリケーション

ECMゲルは、上皮細胞、内皮細胞、筋肉細胞、神経細胞および腫瘍細胞に使用できます。

生物化学的/生理学的作用

ECMゲルは、20~40℃になると熱活性型の重合を受け、再構成基底膜を形成します。 PC12細胞は、このECMゲルの薄層上で増殖すると2日以内に神経突起形成を示します。

構成

ECMは、主成分としてのラミニン、IV型コラーゲン、ヘパリン硫酸プロテオグリカン、エンタクチン、その他の微量成分を含有し、タンパク質濃度が8~12 mg/mL(DMEM中)となるように調製されました。ECMゲルにIV型コラーゲンを添加すると、重合が増加します。

製品には、50 mg/L硫酸ゲンタマイシンが含有されています。

注意

長期間保存する場合は、製品を-20℃に維持してください。ECMゲルは、2~8℃で72時間まで保存できます。

調製ノート

この製品は、マウスのEngelbreth-Holm-Swarm肉腫から調製されます;クロロホルムに対して透析。ゲルは、使用前に2~8℃で一晩解凍したのち、2~8℃に予冷したプレートとピペットを使用してマルチウォールプレートに分注する必要があります。ゲルは、2~8℃のDulbecco改変Eagle培地で最大2倍に希釈可能で、ゲルをプレートに加える前に行う必要があります。 本製品は、20℃で5分以内にゲル化します。長時間操作する場合は、10℃未満で作業を行う必要があります。細胞は、0.5 mmの薄いゲル層の表面に播種するか、または1 mm層の中で培養できます。 層内で細胞を培養する場合は、1 mLあたり3~4 x 104細胞の推奨密度で播種する前に、細胞をゲルに添加します。 このゲルから細胞を解離するためには、カルシウム、マグネシウム、またはEDTAを含有していないプロテアーゼのPBS溶液を、1 mLあたり0.6~2.4単位の濃度で使用します。

保管分類コード

12 - Non Combustible Liquids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable

試験成績書(COA)

ロット番号を入力して試験成績書(COA)を検索してください。

原産地証明書(COO)

ロット番号を入力して原産地証明書(COO)を検索してください。

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. The data sheet for Product E1270, ECM Gel from Engelbreth-Holm-Swarm murine sarcoma, says that it may be diluted up to twofold. What does that mean?

    ECM gel can be diluted twofold with cold Dulbecco's Modified Eagle's Medium (DME).  This means 1 part ECM gel and 1 part DME.

  4. Are ECM Gels, Products E1270 and E6909, the same as Matrigel (from BD)?

    Product No. E1270, ECM gel is the equivalent of Matrigel from BD only produced by Sigma-Aldrich.

  5. Are Products E1270 and E6909, ECM Gel from Engelbreth-Holm-Swarm murine sarcoma, growth factor reduced like Matrigel (from BD)?

    Product No. E1270 is not growth factor reduced. Product No. E6909 is ECM gel, growth factor reduced.

  6. Do Products E1270 and E6909, ECM Gel from Engelbreth-Holm-Swarm murine sarcoma, contain phenol red?

    Product E1270 ECM gel does contain phenol-red from the Dulbecco's modified Eagle's medium component of the product.  Product No. E6909, ECM gel, growth factor reduced, is phenol red free.

  7. What is the calcium concentration in Products E1270, ECM Gel from Engelbreth-Holm-Swarm murine sarcoma?

    The approximate concentration of CaCl2 in the ECM solution is 0.265 mg/mL.

  8. How can I retrieve my cells from Product E1270,  ECM Gel from Engelbreth-Holm-Swarm murine sarcoma?

    Dispase (Product No. D4818) is a Bacillus-derived neutral protease that is recommended for recovering cells cultured on ECM (Product No. E1270) or Matrigel. Dispase will yield a single cell suspension far more effectively than trypsin, collagenase, or other proteolytic enzymes. Dispase is gentle and will not damage cells harvested for sub-cultivation or other study.

  9. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  10. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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資料

ECMゲルマトリックスEHS基底膜抽出物を用いたプロトコル集

私たちのECMゲルマトリックスEHS基底膜抽出物(BME)を用いた、神経突起伸長、細胞浸潤および血管新生時の管腔形成細胞培養プロトコル集

ECM Gel Matrix: Protocols Using EHS Basement Membrane Extracts

Cell culture protocols using ECM Gel Matrix EHS basement membrane extract (BME) for neurite outgrowth, cell invasion and angiogenesis tube formation assays.

Angiogenesis Assays

Cell based angiogenesis assays to analyze new blood vessel formation for applications of cancer research, tissue regeneration and vascular biology.

Controlled Fabrication Methods for Tissue Engineering Constructs

Highlighting existing and novel fabrication methods for both, solid and hydrogel-based scaffold for tissue engineering applications.

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