Heat inactivation is required based on the specific application or protocol. It is typically necessary for hybridoma cultures or immunological studies that require the inactivation of complement proteins. However, heat inactivation is not required for all applications.
F2442
ウシ胎児血清
USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma
別名:
FBS, FCS, 血清
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この商品について
Biological source:
bovine fetus
Origin:
USA origin
Sterility:
sterile-filtered
Shipped in:
dry ice
スキップする
biological source
bovine fetus
sterility
sterile-filtered
product line
FBS Classic
composition
Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL
origin
USA origin
technique(s)
cell culture | hybridoma: suitable, cell culture | mammalian: suitable
impurities
≤10 EU/mL endotoxin
shipped in
dry ice
storage temp.
−20°C
Quality Level
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当該品目 | |||
|---|---|---|---|
| origin USA origin | origin USA origin | origin USA origin | origin Australia origin |
| sterility sterile-filtered | sterility - | sterility sterile-filtered | sterility sterile-filtered |
| biological source bovine fetus | biological source bovine fetus | biological source bovine fetus | biological source bovine fetus |
| technique(s) cell culture | hybridoma: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | hybridoma: suitable, cell culture | insect: suitable, cell culture | mammalian: suitable | technique(s) cell culture | hybridoma: suitable, cell culture | mammalian: suitable |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin |
General description
ウシ胎児血清（FBS）は、最適な増殖促進および増殖抑制機能を示す、低分子および高分子量生体分子の複雑な混合物です。FBSは、成長因子、タンパク質、ビタミン、微量元素、ホルモンなどの分子を含んでいます。これらの分子は、細胞の増殖や維持に重要です。細胞培養培地のための一般的なサプリメントです。FBSは、ウシ胎児血液由来です。FBSは、フィブリンと凝固因子が除去されています。FBSの熱不活化により、補体成分が不活化されます。凝固血由来のFBSは、真核生物、特に哺乳類の細胞培養において、最も幅広く使用される非定義のサプリメントです。[1]1950年代から60年代にかけて開発された旧知の培地に、一般的に使用されます。これらには、最小必須培地（MEM）；[2]ダルベッコ変法イーグル培地（DME、DMEM）；イスコフ改変イーグル培地（IMDM）；[3]M199[4]およびロズウェルパーク記念研究所培地（RPMI-1640）などの多くの一般的な培地が含まれます。[2]
Application
Biochem/physiol Actions
FBSは、細胞培養に幅広い機能を提供するので、依然として定評のある培地サプリメントです。FBSは、栄養、増殖因子および付着因子を送達し、無血清培地（SFM）系では再現困難なメカニズムにより細胞を酸化的損傷とアポトーシスから防御します。
Analysis Note
- エンドトキシンおよびヘモグロビン試験済み
- 細菌、ウイルス、マイコプラズマの存在の有無について検査済み
- 無菌条件下にて0.1ミクロン膜で3回ろ過済みです。
Other Notes
細菌、ウイルス、マイコプラズマ、エンドトキシンの存在の有無について検査済みであり、ヘモグロビン含量についても検査済みです。無菌条件下にて0.1ミクロン膜で3回ろ過済みです。
Related product
製品番号
詳細
価格
保管分類
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
F2442-PH: + F2442-20ML-J: + F2442-500ML: + F2442-100ML: + F2442PROC: + F2442-SAMPLE-J: + F2442-1L: + F2442-500ML-CO: + F2442-100ML-CO: + F2442-6X500ML: + F2442-VAR: + F2442-24X500ML: + F2442-50ML-J: + F2442-BULK: + F2442-50ML: + F2442-ML: + F2442-BULKBWY:
jan
Axenic Procyclic Culture of L. tropica and L. donovani in Culture of FBS-free Medium
Mosa FM, et al.
Ife Journal of Science, 4B, 3088-3093 (2015)
Inhibiting tyrosine phosphorylation of protein kinase Cd (PKCd) protects the salivary gland from radiation damage.
Wie SM et al.
The Journal of Biological Chemistry, 289, 10900-10900 (2014)
MENT, a heterochromatin protein that mediates higher order chromatin folding, is a new serpin family member.
Grigoryev SA et al.
The Journal of Biological Chemistry, 274, 5626-5626 (1999)
Cunzhi Yu et al.
Frontiers in pharmacology, 8, 832-832 (2017-12-07)
Low solubility, tissue accumulation, and toxicity are chief obstacles to developing triptolide derivatives, so a better understanding of the pharmacokinetics and toxicity of triptolide derivatives will help with these limitations. To address this, we studied pharmacokinetics and toxicity of (5R)-5-hydroxytriptolide
Optimization of chemically defined cell culture media--replacing fetal bovine serum in mammalian in vitro methods.
van der Valk J et al.
Toxicology in vitro, 24, 1053-1053 (2010)
資料
FBSおよびその他の血清製品に関するよくあるお問い合わせ（FAQ）への回答および関連製品のリンクはこちら。
細胞培養にウシ胎児（または子ウシ）血清を使用する際の基本と、調達や規制が細胞アッセイ用血清の価格にどのように影響するのかご確認ください。
プロトコル
Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.
Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.
Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.
安定で長期的な遺伝子サイレンシングおよび表現型の変化を達成するための、MISSION shRNAレンチウイルス粒子の形質導入（トランスダクション）の詳細な手順
関連コンテンツ
Our broad range of the most trusted tools for cell culture includes stringently sourced and tested FBS, established media formulations, and sterile labware. Cutting-edge techniques using stem cells and 3D matrices are enabled by organoids, hydrogels, culture scaffolds, and bioinks for 3D bioprinting.
Products and support for mpox research, offering world-class reagents for immunoassay and molecular assay development.
Step-by-step protocol for generating apical-out human gut organoids for microbiome, ADME/Tox, viral and gastrointestinal related disease research. See the complete organoid culture protocol.
メルクのウシ胎児血清（FBS）のポートフォリオから、お客様のニーズとご予算に合う最適なFBSをお探しください。血清サンプルのご依頼。
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is heat inactivation necessary?
1 回答-
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How to heat inactivate this serum Fetal Bovine Serum USA origin,(Cat Log - F2442)
1 回答-
Heat inactivation occurs when serum is heated to 56°C for a period of 30 minutes.
Here's a protocol for heat inactivation:
1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.
2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.
3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.
4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Sigma-Aldrich does not recommend repeated thaw-freeze cycles.役に立ちましたか？
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is this Heat Inactivate FBS?
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This product has not been heat inactivated.
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can F2442 FBS be used for culturing peritoneal macrophages? what is the suitable one?
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This product is tested for suitability in mammalian cell culture. While it is not specifically tested on macrophages, it is subject to stringent specifications, including low endotoxin, and should perform well. Please see the link below to review a sample Certificate of Analysis:
https://www.sigmaaldrich.com/coa/SIGMA/F2442/23A317役に立ちましたか？
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Can this f2442 fbs be used for culture expansion of mesenchymal stem cells? Please confirm Thanks
1 回答-
Fetal Bovine Serum (Cat. no.: F2442) is suitable for use in mammalian cell culture. However, it is not tested specifically in mesenchymal stem cell culture. There is some evidence in the literature that this product would be suitable. See the link below for a relevant reference:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4545441/役に立ちましたか？
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Does Sigma-Aldrich offer fetal bovine serum (FBS) that is antibiotic-free?
1 回答-
FBS can be tested for the presence of antibiotics. Products F2442, F0926, and F6178, Fetal Bovine Serum are routinely tested for Tetracycline (by far the most commonly used antibiotic in animals) and the results are reported on the lot specific Certificate of Analysis.Per the USDA, Antibiotics may be given to prevent or treat disease in cattle. A "withdrawal" period is required from the time antibiotics are administered until it is legal to slaughter the animal. This is so residues can exit the animal's system. FSIS randomly samples cattle at slaughter and tests for residues. Data from this Monitoring Plan have shown a very low percentage of residue violations.The USDA does random sampling of all cattle submitted for slaughter in USDA inspected facilities for antibiotics. The mandatory withdrawal period is 24 hours. Based on withdrawal periods required by the USDA, animal testing at slaughter and finished product testing, our serum is antibiotic free.
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Are all Fetal Bovine Serum (FBS) products heat inactivated?
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Not all Fetal Bovine Serum products are heat inactivated. Product numbers F4135 and 12306C are heat-inactivated FBS.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is Fetal Bovine Serum, Product F2442, available as a charcoal stripped, de-lipidated serum?
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We offer Product No. F6765 (Fetal Bovine Serum, charcoal stripped). The charcoal stripping decreases the levels of hormones; it may also decrease the levels of lipids. If further reduction of lipid levels is required, you may need to treat the serum with fumed silica (e.g. Product No. S5130). Use 20 grams of S5130 per Liter of serum (not plasma). It is mixed for 3-4 hours, then centrifuged in 1 L containers for about 15 minutes at 2000 xg. The supernatant can then be decanted, and filtered if desired. NOTE: Silica is extremely fluffy, and care must be taken not to inhale its dust. Allow for about 15% loss of the serum due to adsorption.
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Does Sigma-Aldrich offer delipidated bovine serum?
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At this time, Sigma-Aldrich does not offer delipidated bovine serum (either FBS or calf serum).A method for lipid depletion, though not necessarily that of Sigma:1. While mixing, gradually add 10 g fumed silica (Product S5130 or Aerosil) per liter of serum2. Mix at room temperature and then transfer to 2-8 °C for overnight mixing.3. After overnight mixing, remove flumed silica (Aerosil) by centrifugation at 3000-5000 rpm for 5 minutes.4. Retain the supernatant. The lipids should stick to the silica. There may be some protein loss
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