H7006

HEPES ナトリウム塩

≥99.0% (titration)

• 別名: 4-(2-ヒドロキシエチル)ピペラジン-1-エタンスルホン酸 ナトリウム塩, N-(2-ヒドロキシエチル)ピペラジン-N′-(2-エタンスルホン酸) ナトリウム塩
• Empirical Formula (Hill Notation): C₈H₁₇N₂NaO₄S
• CAS番号: 75277-39-3
• 分子量: 260.29
• EC Number: 278-169-7
• MDL番号: MFCD00036463
• PubChem Substance ID: 24895780
• NACRES: NA.25

特性

• 品質水準: 400
• アッセイ: ≥99.0% (titration)
• 形状: powder
• pH: 10-12 (1 M in water)
• useful pH range: 6.8-8.2
• pKa (25 °C): 7.5
• 溶解性: water: 0.1 M, clear, colorless
• application(s): diagnostic assay manufacturing
• 保管温度: room temp
• SMILES string: [Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O
• InChI: 1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
• InChI key: RDZTWEVXRGYCFV-UHFFFAOYSA-M

詳細

詳細

HEPES salt, or 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, is a zwitterionic biological buffer that is widely used in biological and biochemical research. It has a buffering range of pH 6.8 to 8.2, making it ideal for a variety of research applications. HEPES is also a popular choice for buffering cell culture media, offering advantages such as lower cell toxicity in comparison to alternatives like Tris and phosphate.

Widely utilized in cell biology and biochemical research, HEPES proves its versatility in protein purification and extraction protocols, owing to its stability across a broad pH range and minimal metal ion binding. Additionally, HEPES proves valuable in various biological and biochemical processes, including immunoprecipitation, cell lysis, and live cell imaging, making it a versatile tool in diverse research applications.

アプリケーション

HEPES buffer finds application in:

• Most studies with metal ions
• Substitute for Tris and phosphate in studies with metal ions
• Grinding buffer in plant studies
• Running buffer in gel electrophoresis
• Buffer in electroporation studies
• Bradford or bicinchoninic acid (BCA) assays
• Extraction buffer to prevent damage of proteins in red blood cells

It has been used:

• as a buffer component in the preparation of a low sucrose buffer
• as a buffer component in a modified Hanks′ solution, a pseudo-physiological solutions

特徴および利点

• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
• Highly soluble in water
• Negligible metal ion binding
• Less toxic to cells than other buffers such as Tris and phosphate
• Stable in a wide pH range

物理的形状

凍結乾燥粉末、噴霧乾燥粉末、または結晶。

その他情報

HEPESナトリウム塩製品を簡単に比較検討できる HEPESナトリウム塩規格表をご参照ください。

安全性情報

• 保管分類コード: 13 - Non Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)