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HYSC020

Sigma-Aldrich

HyStem®-C Cell Culture Scaffold Kit(細胞培養スキャフォールドキット)

For 7.5 mL of hydrogel scaffold solution

NACRES:
NA.75

品質水準

100

保管温度

−20°C

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詳細

The HyStem® -C Cell Culture Scaffold Kit provides an excellent starting point for optimizing the matrix for stem cell culture. It is recommended for cultures, which require a minimal number of cell attachment sites or the addition of other extracellular matrix (ECM) proteins.

アプリケーション

HyStem® -C cell culture scaffold kit has been used for neural stem cell culture.

特徴および利点

Hystem-C enables the attachment of a wide variety of cell types through Gelin-S (thiolated gelatin).

With Hystem-C, researchers can tailor the incorporation of ECM proteins and growth factors as well as the elasticity of the hydrogel and cell incorporation (encapsulations or top plating) to create the specific 3D environment required by their cells.

Hystem-C has been successfully used to expand human embryonic stem cells, human mesenchymal stem cells, neural progenitor cells and hepatic progenitor cells.
Hystem-Cハイドロゲル細胞培養スキャフォールドは、カスタマイズが可能であると同時に十分なキャラクタライズと一貫性が実現された製品であり、細胞がin vivoのように増殖できる3次元環境を提供します。
・カスタマイズ可能-多細胞生物の各種細胞は、それぞれが独自の微小環境を有しています。Hystem-Cは、in vivoと同様の細胞培養環境を作り出すための柔軟性があり、増殖や分化を最適化します。
・合成マトリックス-Hystem-Cは合成マトリックスであることから、抽出由来で成分のあいまいなECM代用物に比べて細胞環境のコントロールに優れています。成分はすべて十分にキャラクタライズされており、組成は一貫しています。Hystem-Cはヒアルロン酸(合成品)、Extralink(合成品)、Gelin-S(ブタ)、水を含有しています。
・生物学的な精密性-ヒアルロン酸と変性コラーゲンが豊富なHystem-Cハイドロゲルによって、生命体に見いだされる細胞の複雑な3次元環境が綿密に再現され、目的の細胞培養に応じた最適な環境を準備することができます。

法的情報

Hystem is a registered trademark of BIOTIME, INC

ピクトグラム

CorrosionExclamation mark

シグナルワード

Danger

危険有害性情報

Hazard Classifications

Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

試験成績書(COA)

原産地証明書(COO)

Douglas O Sobel et al.
Islets, 12(4), 71-86 (2020-06-23)
Immortalized beta cells are an abundant source of insulin-producing cells. Although MIN-6 cells have similar characteristics as normal islets in vitro, the in vivo use of MIN-6 cells has not been fully described. This study characterizes in vivo mouse models...
Reem Basuodan et al.
Journal of anatomy, 233(2), 155-166 (2018-05-11)
Human neural stem cells (hNSC) derived from induced pluripotent stem cells can be differentiated into neurons that could be used for transplantation to repair brain injury. In this study we dispersed such hNSC in a three-dimensional artificial extracellular matrix (aECM)...
Human neural stem cells dispersed in artificial ECM form cerebral organoids when grafted in vivo
Basuodan R, et al.
Journal of Anatomy (2018)
Human neural stem cells dispersed in artificial ECM form cerebral organoids when grafted in vivo
Basuodan R, et al.
Journal of Anatomy (2018)

資料

What is a Cell Delivery Vehicle?

Cell delivery vehicle are components that can be associated with cells and that allow transplantation in human or animals hosts. These can be natural or synthetic components that will form a gel, embedding the cells.

Heparin Versus Heparin Sulfate Proteoglycans

Heparin is distinct from heparan sulfate proteoglycans (HS) which is primarily produced by mast cells. While, HS is produced by all cell types. Know more about the difference between heparin and heparin sulfate proteoglycan and select the right component for your study.

What is Hyaluronan?

Hyaluronan is a major component of the extracellular matrix. It’s the simplest glycosaminoglycan, and it plays several biological roles.

HyStem™ Hyaluronic Acid Based Hydrogels for 3D Cell Culture Applications

Hystem 3D hydrogels are based on hyaluronic acid, a major component of the extracellular matrix. 3D cell culture can be done using Hystem hydrogels.

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