Merck
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I2911

Sigma-Aldrich

StableCell IMDM

With stable glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):
イスコフ改変ダルベッコ培地, IMDM
MDL番号:

品質水準

無菌性

sterile-filtered

形状

liquid

technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

不純物

Endotoxin, tested

構成

NaHCO3: yes
glucose: yes
stable glutamine: yes
phenol red: yes

輸送温度

ambient

保管温度

2-8°C

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This Item
M0446D0697R2405
Sigma-Aldrich

Sigma-Aldrich

I2911

StableCell IMDM

Sigma-Aldrich

Sigma-Aldrich

R2405

RPMI-1640培地

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, tested

impurities

endotoxin, tested

impurities

Endotoxin, tested

impurities

endotoxin, tested

components

NaHCO3: yes
glucose: yes
stable glutamine: yes
phenol red: yes

components

sodium pyruvate: no
HEPES: no
Hanks’ salts (2% CO2): no
NaHCO3: yes
Earle’s salts (5% CO2): no
stable glutamine: yes
phenol red: yes
L-glutamine: no
glucose: yes

components

glucose: high
sodium pyruvate: yes
stable glutamine: yes
NaHCO3: yes
phenol red: yes
HEPES: yes

components

sodium pyruvate: no
NaHCO3: yes
phenol red: yes
stable glutamine: yes
HEPES: no

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

詳細

本IMDMは、L-アラニル-L-グルタミンジペプチドを補充しているため、当初の処方品とは異なります。グルタミンは細胞培養で不安定であることが知られています。本ジペプチドは、細胞培養でより安定型のグルタミンを提供します。

アプリケーション

Guilbertおよびイスコフは、既知組成の無血清培地にアルブミン、トランスフェリン、レシチン、セレンを補充することにより赤血球やマクロファージの前駆細胞を培養可能であることを明らかにしました。本培地は、ダルベッコ改変イーグル培地(DME)を改変し、セレン、追加のアミノ酸およびビタミン、ピルビン酸ナトリウム、HEPESバッファー、硝酸鉄に代わり硝酸カリウムを含有しています。さらに研究を進めた結果、イスコフ培地は、マウスBリンパ球、骨髄由来造血組織、リポポリサッカリドの刺激を受けたB細胞、Tリンパ球、様々なハイブリッド細胞を支持することが明らかにされました。

法的情報

StableCell is a trademark of Sigma-Aldrich Co. LLC

保管分類コード

12 - Non Combustible Liquids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable


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