This product can be resuspended in PBS instead of water. Both water and PBS are suitable solvents, and a hazy solution will form in either.
L2630
リポポリサッカリド from Escherichia coli O111:B4
purified by phenol extraction
Lipopolysaccharides from Escherichia coli O111:B4
別名:
LPS
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製品名
リポポリサッカリド from Escherichia coli O111:B4, purified by phenol extraction
biological source
Escherichia coli (O111:B4)
form
lyophilized powder
purified by
phenol extraction
technique(s)
ELISA: suitable
cell based assay: suitable
ligand binding assay: suitable
impurities
≤3.00% protein (Lowry-TCA)
color
off-white to faint yellow
solubility
water: soluble
application(s)
cell analysis
clinical research
life science and biopharma
shipped in
ambient
storage temp.
2-8°C
Quality Level
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1 of 4
当該品目 | L2880 | L3129 | L3012 |
|---|---|---|---|
| technique(s) ELISA: suitable, ligand binding assay: suitable, cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) - | technique(s) - |
| biological source Escherichia coli (O111:B4) | biological source Escherichia coli (O55:B5) | biological source Escherichia coli (O127:B8) | biological source Escherichia coli (O111:B4) |
| Quality Level 300 | Quality Level 300 | Quality Level 200 | Quality Level 300 |
| solubility water: soluble | solubility water: soluble | solubility water: soluble | solubility water: soluble |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
Application
リポ多糖（LPS）は、グラム陰性菌の細胞壁に特徴的な成分です。LPSとその成分である脂質Aは、一般的な病原体関連分子パターン（PAMP）を認識するトール様受容体タンパク質ファミリーの1つである、トール様受容体4（TLR4）による先天的な免疫システムの細胞を刺激します。
Biochem/physiol Actions
リポ多糖（LPS）は、外壁の一部としての役割を果たすことにより、抗菌物質などの疎水性分子が外壁を透過できるようにして、グラム陰性菌を本質的に耐性にします。LPSは、宿主系の反応を調節する細菌-宿主相互作用において機能を果たします。[7]
General description
リポ多糖（LPS）は糖脂質であり、脂肪酸と結合した糖質で構成されます。[7]リポ多糖（LPS）は、グラム陰性菌の細胞壁に特徴的な成分です。典型的なLPS分子は、脂質A（グルコサミンベースのリン脂質）を持ち、比較的短いコアオリゴ糖と末端の多糖（O-抗原）を持っています。LPSは、外膜のインテグリティに寄与し、細胞を胆汁酸塩や親油性抗生物質の作用から保護します。TLR2およびTLR4（トル様受容体）を介して宿主細胞を刺激します。この刺激は、TNFα（腫瘍壊死因子）、IL-1（インターロイキン1）、およびIL-6などのさまざまな炎症誘発性サイトカインの産生を引き起こします。[8][9]
本製品は、大腸菌セロタイプO111:B4からフェノール抽出されました。元となる株は、個人所有です。このLPSセロタイプは、B細胞の刺激や、ヒト肝細胞におけるNOS（一酸化窒素合成酵素）の誘導に使用されています。
本製品は、大腸菌セロタイプO111:B4からフェノール抽出されました。元となる株は、個人所有です。このLPSセロタイプは、B細胞の刺激や、ヒト肝細胞におけるNOS（一酸化窒素合成酵素）の誘導に使用されています。
Other Notes
Preparation Note
本製品は、水（5 mg/mL）または細胞培養培地（1 mg/mL）に可溶であり、非透明で薄黄色の溶液を与えます。より高濃度（20 mg/mL）の溶液は、生理食塩水に溶解し、ボルテックスおよび70～80oCに加温することで作成できます（透明にはなりません）。リポ多糖は、あらゆる溶媒中でミセルを形成する分子です。濁った溶液が、水やリン酸緩衝食塩水の溶液で得られます。有機溶媒でも、より透明な溶液は与えません。メタノールは、浮遊物のある濁った懸濁液を与え、一方、水は均一な濁った溶液を与えます。
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詳細
価格
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 2 Oral
保管分類
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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資料
An overview of human microbiome research, workflow challenges, sequencing, library production, data analysis, and available microbiome reagents to support your research.
Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.
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Can I resuspend this LPS in PBS instead of water, without hindering its performance ?
1 回答-
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf役に立ちましたか？
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When I compared COAs of two batches of LPS (L2630) we have been using, I realized the potency of the newer one is 10-fold higher than the older one (30000000 and 3000000 EU/mg). Is it common for LPS that different lots have such different potency?
1 回答-
Significant lot-to-lot variability in potency is quite normal for this product. It is a somewhat crude phenol extract; it is not chromatographically purified. That is, a chromatographically purified LPS such as Product L3012 would be expected to have better lot-to-lot consistency. Note that for both this product and L3012, the potency specification is greater than or equal to 500,000 Endotoxin units per milligram (EU/mg); there is a lower limit, but there is no upper limit.
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Is there any harm in filter sterilizing a solution with this LPS before using it in cell culture or animals? Meaning does it change or lose potency if it is sterile filtered say through a 0.2 micron filter?
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Sterile filtration should not affect the concentration or properties of the solution if the LPS is fully dissolved, but please keep in mind that this is a research-grade material that has not been best tested on animals. For a cell culture grade material that has already been γ-irradiated, please consider the following: https://www.sigmaaldrich.com/product/sigma/l4391
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I received LPS L2630 at Room Temperature. Is it ok to keep it at Room Temperature before reconstitution, although recommendations are to store it between 2-8 C?
1 回答-
This product ships routinely at ambient temperature and is stable up to 2 weeks to allow for transit time. The item is to be stored at 2-8°C when received.
Please see the link below to review the product storage and shipping guidelines:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/232/426/storage-transport-conditions-ms.pdf役に立ちましたか？
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Is product L2630 suitable for use in mammalian cell culture?
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Specific testing or validation for the use of product L2630 in mammalian cell culture has not been conducted.
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Hi i recieved L2630 10mg Lipopolysaccharides from Escherichia coli O111:B4 i will used in experimental work. how i will dilute it
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The product is soluble in water (5 mg/mL) or cell culture medium (1 mg/mL). Please see the link below to review additional preparation and storage information:
https://www.sigmaaldrich.com/US/en/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharides役に立ちましたか？
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What is the purity level of this product ? is it ultrapure? 100% pure LPS ?
1 回答-
This product is not tested for purity. However, it is tested for potency on a lot-specific basis.
Kindly review a sample CoA accessible at this link: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/L2630-VAR0000271356.pdf
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Once a solution is developed by dissolving product Lipopolysaccharides from Escherichia coli O111:B4 in water, what is the storage protocol and and how long will it remain usable?
1 回答-
Please see the following link describing our LPS products: https://www.sigmaaldrich.com/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharides
STORAGE/STABILITY
Solutions at 1 mg/mL in buffer or culture medium are stable for approximately one month at 2-8 °C. Frozen aliquots can be stored up to 2 years. Repeated freeze/thaw cycles are not recommended. Solutions should be stored in silanized containers, since LPS can bind to plastics and certain types of glass (especially at concentrations of <0.1 mg/mL). If the LPS concentration is >1 mg/mL, adsorption to the sides of the vial is negligible. If glass containers are used, solutions should be vortexed for at least 30 minutes to redissolve the adsorbed product.役に立ちましたか？
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