This product is not sterile. Stock solutions may be filter-sterilized using a low protein binding membrane, such as PES (Polyethersulfone) membrane. Please see the link below to review product L6529, which is a sterile powder.
https://www.sigmaaldrich.com/product/sigma/l6529
L2880
リポポリサッカリド 大腸菌055:B5由来
purified by phenol extraction
別名:
LPS
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| サイズ/SKU | 在庫状況 | 単価 |
|---|---|---|
10 mg | 在庫あり出荷可能fromsìså¬368 | ￥17,800 |
25 mg | 在庫あり出荷可能fromsìså¬368 | ￥31,000 |
100 mg | 在庫あり出荷可能fromsìså¬368 | ￥74,200 |
500 mg | 在庫あり出荷可能fromsìså¬368 | ￥261,000 |
この商品について
biological source
Escherichia coli (O55:B5)
Quality Segment
form
lyophilized powder
purified by
phenol extraction
technique(s)
cell based assay: suitable
impurities
<3% Protein (Lowry)
color
off-white to tan
solubility
water: soluble
application(s)
cell analysis
clinical research
life science and biopharma
shipped in
ambient
storage temp.
2-8°C
General description
Application
大腸菌O55:B5由来のリポ多糖は以下に使用されています：
リポ多糖（LPS）類はグラム陰性細菌の細胞壁に特徴的な化合物です。LPSおよびそのリピド A 部分は、共通の病原体関連分子構造を認識する Toll 様受容体タンパク質ファミリー（PAMPs）の一員である Toll 様受容体 4（TLR4）により、自然免疫系細胞を刺激します。
Biochem/physiol Actions
Other Notes
LPS 製剤を最適に再構成するためには、容器の種類にかかわらず、使用前に集中的なボルテックスが必要です。保存液の濃度が少なくとも 1 mg/ml であることを確認してください。ホウケイ酸（シラン処理）容器が推奨されますが、ポリプロピレン（PP）プラスチック容器を使用することもできます。
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当該品目 | |||
|---|---|---|---|
| description purified by phenol extraction | description purified by trichloroacetic acid extraction | description FITC conjugate | description Ready Made solution, 1 mg/mL |
| technique(s) cell based assay: suitable | technique(s) - | technique(s) - | technique(s) - |
| biological source Escherichia coli (O55:B5) | biological source Escherichia coli (O55:B5) | biological source Escherichia coli (O55:B5) | biological source Escherichia coli (O55:B5) |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| solubility water: soluble | solubility water: soluble | solubility H2O: 4.90-5.10 mg/mL, faintly hazy to hazy, pale yellow to yellow | solubility water: 4.90-5.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form aqueous solution |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
Still not finding the right product?
Explore all of our products under リポポリサッカリド 大腸菌055:B5由来
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 2 Oral
保管分類
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
L2880-25MG: + L2880-500MG-PW: + L2880-10MG: + L2880-BULK: + L2880-100MG-PW: + L2880-100MG: + L2880-25MG-PW: + L2880-500MG: + L2880-VAR: + L2880-PM: + L2880-10MG-PW:
jan
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Is the product sterile or would you recommend filtration before use in cells?
1 回答-
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How can I determine the shelf life / expiration / retest date of this product?
1 回答-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf役に立ちましたか？
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I recently purchased this item and I would like to understand how lethal/toxic/dangerous is for humans once diluted to 0.5mg/ml. I would need to inject ip fish with a certain volume from that stock solution.
1 回答-
Toxicological information, when available, is provided in section 11 of the safety data sheet. It is unclear which fish model is being used to study toxicity. Consulting scientific literature is recommended for these details. Below is some information that may be useful to address the question.
https://www.sigmaaldrich.com/sds/sigma/l2880?userType=anonymous
https://www.tandfonline.com/doi/abs/10.1080/10934529.2012.695254
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 回答-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Hi, Can you provide the information regarding molecular weight of this LPS?
1 回答-
The molecular weight of this product has not been determined. In general, the molecular weight of native LPS may range from 1 – 4 million Daltons or higher. When treated with SDS and heat, the molecular weight of LPS may range from 50 to 100 kDa.
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What is the number of acetyl groups attached to the lipid A moiety?
1 回答-
The acyl chain length and number of acyl groups may vary between bacterial species. Certain researchers claim there are 6 acyl groups for LPS from this strain of bacteria. Please refer to those peer reviewed papers below for more information.
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Can you provide information on the solubility and solution stability of this product?
1 回答-
LPS products in solution are expected to be stable in phosphate buffered saline at a concentration of 1 mg/mL when stored at 4 °C for at least one month. For long-term storage, it is recommended to use frozen aliquots. In order to prevent LPS binding to glass, it is advisable to store the solution in silanized containers. If glass containers are used, it is recommended to vortex the solutions for at least 30 minutes to dissolve any bound LPS.
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Does the bacterial strain have specific information available, such as whether it originates from a private collection or if there is an associated ATCC number?
1 回答-
Unfortunately, the specific strains used to make L2880 are considered proprietary.
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hello, I was wondering what the best way to reconstitute this LPS is
1 回答-
LPS products can be reconstituted in phosphate buffered saline at 1 mg/mL.
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Which LPS grade should be used to induce neurotoxicity in rats?
1 回答-
There is no Lipopolysaccharide product offered specifically for the induction of neurotoxicity in rats. Escherichia coli strain O55:B5 has been used to induce oligodendrocyte toxicity to evaluate the neuroprotective effects of certain compounds. In addition, Escherichia coli strain O111:B4 has been cited in the literature as suitable for LPS-induced neurotoxicity. For use with animal models, LPS purified by gel filtration is most commonly used to eliminate the risk of any residual solvents.
Please see the links below to review the E. coli strains O111:B4 and O55:B5 that have been purified by gel filtration:
Lipopolysaccharides – Glycobiology
https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/research-and-disease-areas/cell-signaling/lipopolysaccharides
Lipopolysaccharides – selection table
https://www.sigmaaldrich.com/US/en/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharides役に立ちましたか？
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