LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose. Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version). For some applications, even lower salt is required (Luria's low salt version).
L2897
寒天入りLBブロス（Lennox）
Highly-referenced microbial growth powder medium with Agar, low salt, suitable for salt-sensitive E. coli culture.
別名:
LBブロス, Luria Bertani アガー
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grade
Molecular Biology
sterility
non-sterile
form
powder
composition
Agar, 15 g/L , NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L
technique(s)
microbiological culture: suitable
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
Quality Level
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当該品目 | L7533 | L7025 | L3022 |
|---|---|---|---|
| form powder | form powder | form tablet | form powder |
| technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable |
| suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms |
| composition Agar, 15 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L , NaCl, 5 g/L | composition Agar, 15 g/L , NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L | composition - | composition NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| application(s) food and beverages | application(s) agriculture | application(s) food and beverages | application(s) food and beverages |
General description
Lennox LBは、E. coliの培養に使用され、頻繁に文献参照される微生物増殖培地です。この栄養豊富な微生物ブロスには、低塩処方でペプチド、アミノ酸および炭水化物が含有されています。
Application
Features and Benefits
Lennox LB粉末は、寒天とともに以下のものをもたらします：
- あらかじめ注がれたプレートに代わる低予算で済む代替品
- より大きなパッケージサイズを用いる簡単なスケールアップ
- 標準処方
Preparation Note
1.35 gを1 Lの蒸留水に懸濁させます。
2.撹拌しながら沸騰するまで加熱することにより、すべての成分を完全に溶解させます。
3.121℃で15分間オートクレーブします。
Lennox L Agarの調製：グルコース1 gを添加し、上記の調製手順に進みます。Enquist and Sternberg培地の調製：オートクレーブ処理後、滅菌1 M硫酸マグネシウム10 mLを無菌操作により添加します。
2.撹拌しながら沸騰するまで加熱することにより、すべての成分を完全に溶解させます。
3.121℃で15分間オートクレーブします。
Lennox L Agarの調製：グルコース1 gを添加し、上記の調製手順に進みます。Enquist and Sternberg培地の調製：オートクレーブ処理後、滅菌1 M硫酸マグネシウム10 mLを無菌操作により添加します。
Legal Information
Zeocin is a registered trademark of Cayla Sarl
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製品番号
詳細
価格
保管分類
11 - Combustible Solids
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flash_point_f
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flash_point_c
Not applicable
ppe
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適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
L2897-VAR: + L2897-1KG:4548173200958 + L2897-250G-PW: + L2897-250G:4548173200965 + L2897-BULK: + L2897-1KG-PW: + L2897-6X1KG:4548173200972
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プロトコル
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関連コンテンツ
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What are the differences among the Luria, Lennox and Miller LB formulations?
1 回答-
役に立ちましたか？
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Will adding magnesium to the culture medium increase cell density?
1 回答-
In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities. You may need to also increase the shaking speed of the incubator.
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
役に立ちましたか？
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Which bacterial culture medium is the best choice for my application?
1 回答-
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
役に立ちましたか？
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What is the difference among the many LB - Lennox products?
1 回答-
LB - Lennox is available in many types to suit your needs. The different product formats include powder, EZMix™ powder, and tablet form. Each of these forms are available with and without agar for easy LB-agar plate preparation.L3022 (LB Lennox powder)L2897 (LB Lennox powder with agar)L7275 (LB Lennox tablets)L7025 (LB Lennox tablets with agar)L7658 (LB Lennox EZMix(TM) powder)L7533 (LB Lennox EZMix(TM) powder with agar)L5542 (LB Lennox pre-poured agar plates)
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