The formulation for L3022 has not changed over the years. The "low salt" label was added later to help customers identify the formulation more easily.
L3022
LBブロス（Lennox）
Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.
LB Broth (Lennox)
別名:
Lennoxブロス, ルリア ベルターニブロス
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grade
Molecular Biology
sterility
non-sterile
form
powder
composition
NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L
technique(s)
microbiological culture: suitable
pH
6.8-7.2(2% solution)
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
Quality Level
1 of 4
当該品目 | L7658 | L7275 | L2897 |
|---|---|---|---|
| technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable |
| suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms |
| form powder | form powder | form tablet | form powder |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| composition NaCl, 5 g/L , Yeast Extract, 5 g/L , Tryptone, 10 g/L | composition - | composition - | composition Agar, 15 g/L , NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L |
| application(s) food and beverages | application(s) agriculture | application(s) food and beverages | application(s) food and beverages |
General description
Lennox LBは、E. coliの培養に使用される、一般的な微生物増殖培地です。この栄養素の豊富な微生物ブロスは、ペプチド、アミノ酸、および糖質を、低塩類組成で含んでいます。
Application
Luriaブロスは、Escherichia coliの維持と繁殖に使用されます。 E. coliは、トリプトンおよびイーストが供給する必須成長因子によって、Luriaブロス中で迅速に増殖します。供給されない場合はE. coliが必須成長因子を合成する必要があります。また、Luriaブロスは、NaCl成分による輸送や浸透圧バランスのための必須電解質を含んでいます。[1]
細胞抽出物のTrimastix分画実験における、T. pyriformis細胞画分の再懸濁に使用されます。[2]
細胞抽出物のTrimastix分画実験における、T. pyriformis細胞画分の再懸濁に使用されます。[2]
クローニング、DNAプラスミド作成、およびリコンビナントタンパク質作成のための、E. coli株の非選択的培養に適しています。 また、ゼオシン®などの低塩濃度が必要なものを含め、適した抗生物質が添加される場合の選択的培養にも適しています。
Features and Benefits
Lennox LB粉末は以下を提供します：
- 秤量不要の便利なスモールパッケージ
- より大きなパッケージサイズによる容易なスケールアップ
- 溶液にかわる低予算な方法
- 標準的な組成
Preparation Note
1.1 Lの蒸留水に20 gを溶かします。
2.121℃で15分間オートクレーブ滅菌します。
Lennox L Brothを調製するには：1 gグルコースを添加してから、上記の調製手順に従います。
EnquistおよびSternbergの培地を調製するには：オートクレーブ後、滅菌した1 M硫酸マグネシウム10 mLを無菌的に添加します。
2.121℃で15分間オートクレーブ滅菌します。
Lennox L Brothを調製するには：1 gグルコースを添加してから、上記の調製手順に従います。
EnquistおよびSternbergの培地を調製するには：オートクレーブ後、滅菌した1 M硫酸マグネシウム10 mLを無菌的に添加します。
Legal Information
Zeocin is a registered trademark of Cayla Sarl
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適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
L3022-BULK: + L3022-VAR: + L3022-6X1KG:4548173201047 + L3022-250G:4548173201030 + L3022-1KG:4548173201023
jan
Francisco Dávila et al.
The Journal of experimental biology, 221(Pt 6) (2018-02-16)
Bacteria can damage sperm and thus reduce the reproductive success of both males and females; selection should therefore favour the evolution of antimicrobial protection. Eusocial hymenopterans might be particularly affected by such bacterial infections because of their mating ecology. In
J Benavente et al.
International journal of biological macromolecules, 103, 758-763 (2017-05-27)
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Margot Doberva et al.
Frontiers in microbiology, 8, 1152-1152 (2017-07-12)
Quorum sensing (QS) is a density-dependent mechanism allowing bacteria to synchronize their physiological activities, mediated by a wide range of signaling molecules including
Zuzana Zubáčová et al.
PloS one, 8(3), e55417-e55417 (2013-03-22)
All eukaryotic organisms contain mitochondria or organelles that evolved from the same endosymbiotic event like classical mitochondria. Organisms inhabiting low oxygen environments often contain mitochondrial derivates known as hydrogenosomes, mitosomes or neutrally as mitochondrion-like organelles. The detailed investigation has shown
Bor Kavčič et al.
Nature communications, 11(1), 4013-4013 (2020-08-13)
Antibiotics that interfere with translation, when combined, interact in diverse and difficult-to-predict ways. Here, we explain these interactions by "translation bottlenecks": points in the translation cycle where antibiotics block ribosomal progression. To elucidate the underlying mechanisms of drug interactions between
プロトコル
General protocols for growth of competent cells in microbial medium.
関連コンテンツ
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Are the new batches of the product labeled as "Low salt" consistent with the older batch that does not have the same label? Consistency in experiments is a concern.
1 回答-
役に立ちましたか？
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
役に立ちましたか？
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Which bacterial culture medium is the best choice for my application?
1 回答-
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
役に立ちましたか？
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Will adding magnesium to the culture medium increase cell density?
1 回答-
In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities. You may need to also increase the shaking speed of the incubator.
役に立ちましたか？
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What is the difference among the many LB - Lennox products?
1 回答-
LB - Lennox is available in many types to suit your needs. The different product formats include powder, EZMix™ powder, and tablet form. Each of these forms are available with and without agar for easy LB-agar plate preparation.L3022 (LB Lennox powder)L2897 (LB Lennox powder with agar)L7275 (LB Lennox tablets)L7025 (LB Lennox tablets with agar)L7658 (LB Lennox EZMix(TM) powder)L7533 (LB Lennox EZMix(TM) powder with agar)L5542 (LB Lennox pre-poured agar plates)
役に立ちましたか？
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What are the differences among the Luria, Lennox and Miller LB formulations?
1 回答-
LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose. Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version). For some applications, even lower salt is required (Luria's low salt version).
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