According to studies carried out on similar products, frozen aliquots could be stable for up to 2 years. It is important to avoid repeated freezing and thawing cycles.
L3024
リポポリサッカリド from Escherichia coli O111:B4
purified by ion-exchange chromatography, TLR ligand tested
別名:
LPS
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由来生物
Escherichia coli (O111:B4)
品質水準
フォーム
lyophilized powder
精製方法
ion-exchange chromatography
不純物
<1% Protein
<1% RNA
色
white to yellow cast
溶解性
water: soluble
輸送温度
ambient
保管温度
2-8°C
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関連するカテゴリー
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当該品目 | L4524 | L4130 | L3129 |
|---|---|---|---|
| biological source Escherichia coli (O111:B4) | biological source Escherichia coli (O55:B5) | biological source Escherichia coli (O111:B4) | biological source Escherichia coli (O127:B8) |
| solubility water: soluble | solubility water: soluble | solubility water: soluble | solubility water: soluble |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| color white to yellow cast | color white to yellow cast | color white to light yellow | color white to faint yellow |
詳細
この製品はE. coli 血清型O111:B4から抽出し、イオン交換で精製します。この細菌株は民間コレクションから得ました。このLPS血清型は、B細胞を刺激しヒト肝細胞でNOS産生を誘導するために使用されてます。
アプリケーション
リポ多糖（LPS）は、グラム陰性菌の細胞壁に特徴的な成分です。LPSとその成分である脂質Aは、一般的な病原体関連分子パターン（PAMP）を認識するトール様受容体タンパク質ファミリーの1つである、トール様受容体4（TLR4）による先天的な免疫システムの細胞を刺激します。
生物化学的／生理学的作用
リポポリサッカライド（LPS）は膜外層に局在しており、非莢膜株では細胞表面に露出しています。LPSは外膜の完全性に寄与し、胆汁酸塩および親油性抗生物質の作用から細胞を保護します。
調製ノート
この製品は水溶性（5 mg/mL）で、細胞培養培地に溶け（1 mg/mL）、濁りを帯びた薄黄色の溶液を生成します。ボルテックスにかけ70～80℃に加温した後、より高濃度の、ただしまだ濁りを帯びた溶液（20 mg/mL）が生理食塩水を溶媒として生成されています。リポポリサッカライドは、あらゆる溶媒でミセルを形成する分子です。水およびリン酸場緩衝生理食塩水で濁りを帯びた溶液が生成されます。有機溶媒では溶液の濁りを取ることはできません。メタノールで浮遊物がある濁った懸濁液が生成されますが、水では均一な濁りが生じます。
関連製品
製品番号
詳細
価格
シグナルワード
Danger
危険有害性情報
危険有害性の分類
Acute Tox. 2 Oral
保管分類コード
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
L3024-10MG-PW:
L3024-25MG:
L3024-BULK:
L3024-25MG-PW:
L3024-5MG-PW:
L3024-5MG:
L3024-10MG:
L3024-VAR:
F T Silvestre et al.
Journal of dairy science, 94(5), 2285-2301 (2011-04-29)
The objectives were to evaluate the effects of differential supplementation of Ca salts (CS) of fatty acids (FA) on plasma acute phase proteins and both FA composition and function (i.e., activity and cytokine production) of neutrophils, during the peripartum and
Carl-Fredrik Johnzon et al.
Frontiers in immunology, 9, 1487-1487 (2018-07-11)
Mastitis is an inflammatory condition of the mammary tissue and represents a major problem for the dairy industry worldwide. The present study was undertaken to study how experimentally induced acute bovine mastitis affects inflammatory parameters and changes in the metabolome.
Yoshitomo Hayama et al.
Journal of immunology (Baltimore, Md. : 1950), 200(11), 3790-3800 (2018-04-25)
Amino acid metabolism plays important roles in innate immune cells, including macrophages. Recently, we reported that a lysosomal adaptor protein, Lamtor1, which serves as the scaffold for amino acid-activated mechanistic target of rapamycin complex 1 (mTORC1), is critical for the
Sharon Elazar et al.
Veterinary research, 41(1), 10-10 (2009-10-16)
Mastitis, inflammation of the mammary tissue, is a common disease in dairy animals and mammary pathogenic Escherichia coli (MPEC) is a leading cause of the disease. Lipopolysaccharide (LPS) is an important virulence factor of MPEC and inoculation of the mammary
Simon N Willis et al.
Nature communications, 8(1), 1426-1426 (2017-11-12)
Humoral immunity requires B cells to respond to multiple stimuli, including antigen, membrane and soluble ligands, and microbial products. Ets family transcription factors regulate many aspects of haematopoiesis, although their functions in humoral immunity are difficult to decipher as a
資料
Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.
関連コンテンツ
Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.
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Wie oft kann aufgelöste LSP E.Coli in Konzentration 1 mg/dL eingefroren und aufgetaut werden?
1 回答-
役に立ちましたか？
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How can I determine the shelf life / expiration / retest date of this product?
1 回答-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf役に立ちましたか？
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 回答-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
役に立ちましたか？
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what is the molecular weight of LPS catalog #L3024?
1 回答-
The molecular weight of this product has not been determined. In general, the molecular weight of native LPS may range from 1 – 4 million Daltons or higher. When treated with SDS and heat, the molecular weight of LPS may range from 50 to 100 kDa.
役に立ちましたか？
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Does the product represent lipid A of LPS with six-acyl chain (hexa-acylated) ?
1 回答-
L3024 is an LPS that contains the lipid A moiety. LPS products that are described as "detoxified" have a lipid A moiety that has been partially delipidated by alkaline hydrolysis. Removal of the fatty acid portions of lipid A results in a detoxified LPS with an endotoxin level about 10,000 times lower than that of the parent LPS. This product is not detoxified.
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