As listed in the 'PROPERTIES' section, the composition in g/L is the following:
Agar, 15 g/L
NaCl, 10 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L
L3147
LBブロス寒天培地（Miller）
Highly-referenced nutrient-rich microbial growth powder medium with Agar, suitable for regular E.coli culture.
別名:
Luria アガー, Miller’s LB アガー
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250 G
￥7,200
1 KG
￥29,330
￥7,200
単価￥14,400割引50%在庫あり出荷可能詳細
この商品について
UNSPSC Code:
41106200
NACRES:
NA.85
eCl@ss:
42040102
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製品名
LBブロス寒天培地（Miller）, Highly-referenced nutrient-rich microbial growth powder medium with Agar, suitable for regular E.coli culture.
grade
Molecular Biology
sterility
non-sterile
form
powder
composition
Agar, 15 g/L
NaCl, 10 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L
technique(s)
microbiological culture: suitable
pH
6.8-7.2(4% solution)
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli
nonselective for coliforms
Quality Level
1 of 4
当該品目 | L3522 | L3272 | L2542 |
|---|---|---|---|
| technique(s) microbiological culture: suitable | technique(s) - | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable |
| suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms |
| form powder | form powder | form powder | form liquid |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility sterile; 0.2 μm filtered |
| composition Agar, 15 g/L , Tryptone, 10 g/L , NaCl, 10 g/L , Yeast Extract, 5 g/L | composition NaCl, 10 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L | composition Agar, 15 g/L , NaCl, 0.5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L | composition - |
| application(s) food and beverages | application(s) food and beverages | application(s) food and beverages | application(s) microbiology |
Application
クローン用大腸菌株の非選択的培養、DNAプラスミド生産、および組換えタンパク質の生産に適しています。 また、適切な抗生物質が加えられたときの選択的培養にも適しています。
Features and Benefits
寒天入りLB粉末（Miller）：
- 大きい包装を使用するとスケールアップが容易です。
- 既製のプレートを低予算で代替できます。
- 標準製剤。
General description
LB（Miller）は、非常によく知られている大腸菌培養に使用される微生物用培地です。 この栄養豊富な微生物用ブロスには、ペプチド、アミノ酸、水溶性ビタミン、及び炭水化物が含まれています。 寒天の添加により、固形の微生物用培地を作成できます。
Preparation Note
1.蒸留水1 Lに40 gを懸濁させます。
2.溶解させるために、かき混ぜながら沸騰させます。
3.121℃で15分間オートクレーブします。
4.50℃まで冷ましてから、滅菌シャーレに分注します。
LuriaおよびBurrowsの培地を調製するには：ブドウ糖1 gを培地に添加してから、上記の調製手順に進みます。
Luria、AdamsおよびTingの培地（LC寒天としても知られる）を調製するには：オートクレーブ後、滅菌0.1 M塩化カルシウム25 mLを無菌的に添加します。
2.溶解させるために、かき混ぜながら沸騰させます。
3.121℃で15分間オートクレーブします。
4.50℃まで冷ましてから、滅菌シャーレに分注します。
LuriaおよびBurrowsの培地を調製するには：ブドウ糖1 gを培地に添加してから、上記の調製手順に進みます。
Luria、AdamsおよびTingの培地（LC寒天としても知られる）を調製するには：オートクレーブ後、滅菌0.1 M塩化カルシウム25 mLを無菌的に添加します。
粉末40 gを1 Lの水に加えます。 撹拌しながら沸騰するまで加熱し、粉末を溶解します。 121℃で15分間オートクレーブし、滅菌します。 少し冷ました後、抗生物質などを添加します（必要に応じて）。
Related product
製品番号
詳細
価格
保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Batch solar disinfection inactivates oocysts of Cryptosporidium parvum and cysts of Giardia muris in drinking water
McGuigan KG, et al.
Journal of Applied Microbiology, 101(2), 453-463 (2006)
Difco Manual, 239-239 (1998)
Multiple microbial activities for volatile organic compounds reduction by biofiltration
Civilini M.
Journal of the Air & Waste Management Association (1995), 56(7), 922-930 (2006)
Miller, J.H.
Experiments in Molecular Genetics, 433-433 (1972)
Kinetic understanding of nitrogen supply condition on biosynthesis of polyhydroxyalkanoate from benzoate by Pseudomonas putida KT2440
Xu Z, et al.
Bioresource Technology, 273, 538-544 (2019)
資料
遺伝子工学により、研究のための遺伝子組換えタンパク質の大規模な発現・単離が可能になります。
Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.
プロトコル
General protocols for growth of competent cells in microbial medium.
関連コンテンツ
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How many grams per liter?? No info on bottle.
1 回答-
役に立ちましたか？
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Can you provide the expiry date for my media? I couldn't find it on the Certificate of Analysis (COA) or the bottle.
1 回答-
The expiry date information can be found on the Certificate of Analysis (COA) or the product packaging. If there is no expiry date information available, your product is covered by a one-year warranty starting from the date it was received at your site.
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There is no instruction on the bottle for LB Broth with agar (L3147-1kg). how much powder medium I should use for making 1L agar?
1 回答-
Preparation instructions for this product are published on the product page under Reconstitution. To reconstitute, add 40g powder to 1 L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired).
役に立ちましたか？
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Will adding magnesium to the culture medium increase cell density?
1 回答-
In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities. You may need to also increase the shaking speed of the incubator.
役に立ちましたか？
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Which bacterial culture medium is the best choice for my application?
1 回答-
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
役に立ちましたか？
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
役に立ちましたか？
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What are the differences among the Luria, Lennox and Miller LB formulations?
1 回答-
LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose. Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version). For some applications, even lower salt is required (Luria's low salt version).
役に立ちましたか？
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What is the difference among the LB - Miller products?
1 回答-
LB - Miller is available in many types to suit your needs. The different product formats include powder and liquid form. The powder form is also available with agar for easy LB-agar plate preparation.L2542 (LB Miller liquid)L3522 (LB Miller powder)L3147 (LB Miller powder with agar)
役に立ちましたか？
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