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M0880

メタンスルホン酸エチル

liquid

Ethyl methanesulfonate

別名:

メシル酸エチル, メタンスルホン酸エチルエステル

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容量カタログ番号在庫状況単価
1 g

在庫あり出荷可能from市川市大町368

¥8,050
5 g

在庫あり出荷可能from市川市大町368

¥10,200
10 g

在庫あり出荷可能from市川市大町368

¥19,200
25 g
カートの在庫状況を確認する
¥32,300
100 g
カートの在庫状況を確認する
¥106,000

この商品について

化学式:
CH3SO3CH2CH3
CAS番号:
分子量:
124.16
UNSPSC Code:
12352108
NACRES:
NA.25
PubChem Substance ID:
EC Number:
200-536-7
Beilstein/REAXYS Number:
773969
MDL number:
Form:
liquid
Quality level:

¥8,050


在庫あり出荷可能詳細


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form

liquid

Quality Level

refractive index

n20/D 1.418 (lit.)

bp

85-86 °C/10 mmHg (lit.)

density

1.206 g/mL at 20 °C

SMILES string

CCOS(C)(=O)=O

InChI

1S/C3H8O3S/c1-3-6-7(2,4)5/h3H2,1-2H3

InChI key

PLUBXMRUUVWRLT-UHFFFAOYSA-N

Application

メタンスルホン酸メチルはマウス胚性線維芽細胞、酵母ヒト胚性腎細胞株およびヒトリンパ球に変異を誘発するために使用されています。

Biochem/physiol Actions

メタンスルホン酸エチルは、DNAエチル化剤であり、植物や動物に対して変異原性で、哺乳類に対して発がん性です。メタンスルホン酸エチルはDNA修復プロセスの研究においてモデルのアルキル化剤として使用されています。EMSはアデニン-チミン(A/T)へのグアニジン-シトシン(G/C)の塩基置換を誘発します。EMSはゲノム中の点変異や一塩基変異多型も発生させます。EMSは米、小麦およびシロイヌナズナに変異を誘発するために使用される潜在的な化学的突然変異原です。

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当該品目
12992516595664296
form

liquid

form

liquid

form

liquid

form

liquid

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

200

density

1.206 g/mL at 20 °C

density

1.3 g/mL at 25 °C (lit.)

density

-

density

1.09 g/mL at 25 °C (lit.)

refractive index

n20/D 1.418 (lit.)

refractive index

n20/D 1.414 (lit.)

refractive index

-

refractive index

n20/D 1.422

bp

85-86 °C/10 mmHg (lit.)

bp

202-203 °C (lit.)

bp

-

bp

103-104 °C/25 mmHg (lit.)


Still not finding the right product?

Explore all of our products under メタンスルホン酸エチル


pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1

保管分類

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

212.0 °F

flash_point_c

100 °C

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

第4類:引火性液体 + 第三石油類 + 危険等級III + 非水溶性液体

fsl

名称等を表示すべき危険物及び有害物

ishl_indicated

名称等を通知すべき危険物及び有害物

ishl_notified

M0880-100G:4.54817394997E12 + M0880-5G:4.548173950013E12 + M0880-10G:4.548173949987E12 + M0880-1G:4.548173949994E12 + M0880-25G:4.548173950006E12

jan



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試験成績書(COA)

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資料

Cancer research has revealed that the classical model of carcinogenesis, a three step process consisting of initiation, promotion, and progression, is not complete.

Cancer research has revealed that the classical model of carcinogenesis, a three step process consisting of initiation, promotion, and progression, is not complete.


Kamel El Omari et al.
Nature communications, 10(1), 846-846 (2019-02-21)
Lipid membrane fusion is an essential function in many biological processes. Detailed mechanisms of membrane fusion and the protein structures involved have been mainly studied in eukaryotic systems, whereas very little is known about membrane fusion in prokaryotes. Haloarchaeal pleomorphic
A Rapid and Precise Mutation-Activated Fluorescence Reporter for Analyzing Acute Mutagenesis Frequency
Birnbaum MD, et al.
Cell Chemical Biology (2018)
The effects of thymol on sister chromatid exchange, chromosome aberration and micronucleus in human lymphocytes
Buyukleyla M and Rencuzogullari E
Ecotoxicology and Environmental Safety, 72(3), 943-947 (2009)



グローバルトレードアイテム番号

カタログ番号GTIN
M0880-10G04061837841064
M0880-1G04061834037521
M0880-5G04061837841071
M0880-100G04061837841057
M0880-25G04061834037538

質問

1–10/13 質問  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 回答
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      役に立ちましたか?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 回答
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      役に立ちましたか?

  3. Does opening a bottle of EMS and thus exposing it to the air accelerates its decomposition? How much time after the first opening can we assume same efficiency? The bottle we got did have a screw cap but no plastic plug. Is that ok?

    1 回答
    1. This product is packaged under argon in an amber glass bottle with a screw cap. This is the standard packaging for this item. The item is indicated as being moisture sensitive and care should be taken to prevent exposure to air to prevent hydrolysis. There is no specific stability study to determine a decomposition timeline, but water from the humidity from air could lead to hydrolysis. Therefore, it is recommended to store the reagent under a dried inert gas such as argon as directed in section 7.2 of the SDS.

      https://www.sigmaaldrich.com/sds/sigma/m0880?userType=anonymous

      役に立ちましたか?

  4. How mM have the EMS solution (5G)?

    1 回答
    1. This product is a neat or pure liquid and has not been diluted in a solvent. This material has a density of 1.206 g/mL and a molecular weight of 124.16 g/mol, with a calculated molarity of 9.71 M. The concentration may be determined as follows:
      M = 1.206 g/mL/124.16 g/mol *1000 = 9.71M

      役に立ちましたか?

  5. Is it suitable to store item M0880, Ethyl methanesulfonate, at 4°C?

    1 回答
    1. The recommended storage condition for item M0880, Ethyl methanesulfonate, is at ambient temperature (>5°C).

      役に立ちましたか?

  6. The bottle has 10g of EMS. How much volume of EMS is present in the bottle? How to make a stock concentration and what are the suggested working concentrations?

    1 回答
    1. Volume =Mass/Density = 10g/1.206 g/ml = 8.29 ml. This product is over-filled up to 5%, so the volume and mass will not necessarily be the exact volume calculated.

      Further, EMS is miscible in chloroform (200 mg/ml). EMS can be diluted to a concentration of 1% in water or serum-free medium and then added to a culture at a final concentration in the range of 200-300 mg/ml:
      https://b2b.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/830/111/m0880pis.pdf

      役に立ちましたか?

  7. Can I make the stock solution 1% in chloroform as the data sheet mentions that the EMS is immiscible in chloroform?

    1 回答
    1. Yes, this product may be prepared as a 1% solution. The 1% solution would be the equivalent of 1g/100mL or 10mg/mL This product is miscible in chloroform at 200 mg/ml. Please see the link below to review the product datasheet for additional information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/830/111/m0880pis.pdf

      役に立ちましたか?

  8. I am using M0880, what should be its dissolving solution, like simply double distilled water or other, for preparing various percentage solution of EMS?

    1 回答
    1. As per the product datasheet, a 1% EMS stock solution can be prepared by diluting in water, serum-free medium, or buffer. The typical working concentration is 200-300 ug/mL. This product is not sterile. Stock solutions should be filter sterilized prior to adding to cultures. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/830/111/m0880pis.pdf

      役に立ちましたか?

  9. How does Product M0880, Ethyl methanesulfonate (EMS), compare to Product 64292, which is the same chemical?

    1 回答
    1. Product No. 64292 is tested for purity by a titration assay. Although M0880 is likely to be of similar quality, there is no purity assay performed on it.

      役に立ちましたか?

  10. Is Product M0880, Ethyl methanesulfonate (EMS), sterile?

    1 回答
    1. It is not necessarily sterile. For those applications that require a sterile EMS solution, the EMS should be added to the appropriate buffer or medium. The buffer or medium should then be sterile-filtered.

      役に立ちましたか?

1–10/13 質問  

レビュー

アクティブなフィルタ

    • 1件のレビュー
    • 0件の投票
    星5/5個です。

    DMSO solution to dissolve EMS

    I am planing to use EMS M0880 for mutagenesis of plant seeds. can I use EMS mixuture with DMSO to prepare a final volume of 1 liter?

    Googleで翻訳する

    役に立ちましたか?

    1. Merck KGaAからの回答:

      We have not tested M0880 for its solubility in DMSO, this would need to be tested in your lab. It is water soluble and can be diluted to a concentration of 1% in water.
      We encourage customers who experience problems with our products to contact us for additional technical support. Please visit https://www.sigmaaldrich.com/US/en/support/customer-support to submit a Product Technical Inquiry.